In placenta, prenatal vitamin intake was marginally associated with -0.52% (95% CI -1.04, 0.01) lower mean array-wide DNA methylation in EARLI, and associated with -0.60% (-1.08, -0.13) lower mean array-wide DNA methylation in MARBLES. There was little consistency in the associations between prenatal vitamin intake and single DNA methylation site effect estimates across cohorts and tissues, with only a few overlapping sites with correlated effect estimates. However, the single DNA methylation sites with p-value < 0.01 (EARLI cord nCpGs = 4068, EARLI placenta nCpGs = 3647, MARBLES cord nCpGs = 4068, MARBLES placenta nCpGs = 9563) were consistently enriched in neuronal developmental pathways.
Together, our findings suggest that prenatal vitamin intake in the first month of pregnancy may be related to lower placental global DNA methylation and related to DNA methylation in brain-related pathways in both placenta and cord blood.
在胎盘中,在EARLI中,产前维生素摄入量与-0.52%(95%CI-1.04,0.01)较低的平均阵列DNA甲基化相关,并与MARBLES中-0.60%(-1.08,-0.13)较低的平均阵列DNA甲基化相关。产前维生素摄入量和单个DNA甲基化位点效应估计在队列和组织之间的关联几乎没有一致性。只有几个重叠的站点具有相关的效果估计。然而,p值<0.01的单个DNA甲基化位点(EARLI脐带nCpGs=4068,EARLI胎盘nCpGs=3647,MARBLES脐带nCpGs=4068,MARBLES胎盘nCpGs=9563)在神经元发育途径中持续富集。
一起,我们的研究结果表明,妊娠首月产前维生素摄入量可能与胎盘整体DNA甲基化水平降低有关,也与胎盘和脐带血大脑相关通路的DNA甲基化有关.