PDF(Pubmed)
Abstract：
Prenatal vitamin use is recommended before and during pregnancies for normal fetal development. Prenatal vitamins do not have a standard formulation, but many contain calcium, folic acid, iodine, iron, omega-3 fatty acids, zinc, and vitamins A, B6, B12, and D, and usually they contain higher concentrations of folic acid and iron than regular multivitamins in the US Nutrient levels can impact epigenetic factors such as DNA methylation, but relationships between maternal prenatal vitamin use and DNA methylation have been relatively understudied. We examined use of prenatal vitamins in the first month of pregnancy in relation to cord blood and placenta DNA methylation in two prospective pregnancy cohorts: the Early Autism Risk Longitudinal Investigation (EARLI) and Markers of Autism Risk Learning Early Signs (MARBLES) studies.
In placenta, prenatal vitamin intake was marginally associated with -0.52% (95% CI -1.04, 0.01) lower mean array-wide DNA methylation in EARLI, and associated with -0.60% (-1.08, -0.13) lower mean array-wide DNA methylation in MARBLES. There was little consistency in the associations between prenatal vitamin intake and single DNA methylation site effect estimates across cohorts and tissues, with only a few overlapping sites with correlated effect estimates. However, the single DNA methylation sites with p-value < 0.01 (EARLI cord nCpGs = 4068, EARLI placenta nCpGs = 3647, MARBLES cord nCpGs = 4068, MARBLES placenta nCpGs = 9563) were consistently enriched in neuronal developmental pathways.
Together, our findings suggest that prenatal vitamin intake in the first month of pregnancy may be related to lower placental global DNA methylation and related to DNA methylation in brain-related pathways in both placenta and cord blood.
In placenta, prenatal vitamin intake was marginally associated with -0.52% (95% CI -1.04, 0.01) lower mean array-wide DNA methylation in EARLI, and associated with -0.60% (-1.08, -0.13) lower mean array-wide DNA methylation in MARBLES. There was little consistency in the associations between prenatal vitamin intake and single DNA methylation site effect estimates across cohorts and tissues, with only a few overlapping sites with correlated effect estimates. However, the single DNA methylation sites with p-value < 0.01 (EARLI cord nCpGs = 4068, EARLI placenta nCpGs = 3647, MARBLES cord nCpGs = 4068, MARBLES placenta nCpGs = 9563) were consistently enriched in neuronal developmental pathways.
Together, our findings suggest that prenatal vitamin intake in the first month of pregnancy may be related to lower placental global DNA methylation and related to DNA methylation in brain-related pathways in both placenta and cord blood.
摘要：
为了正常的胎儿发育，建议在怀孕前和怀孕期间使用产前维生素。产前维生素没有标准配方，但是许多含有钙，叶酸,碘，铁,omega-3脂肪酸，锌，还有维生素A,B6、B12和D，通常它们含有比美国常规复合维生素更高浓度的叶酸和铁。但是母体产前维生素使用与DNA甲基化之间的关系研究相对不足。我们在两个前瞻性妊娠队列中检查了妊娠第一个月的产前维生素与脐带血和胎盘DNA甲基化的关系：早期自闭症风险纵向调查（EARLI）和自闭症风险学习早期体征标志物（MARBLES）研究。
在胎盘中，在EARLI中,产前维生素摄入量与-0.52%(95%CI-1.04,0.01)较低的平均阵列DNA甲基化相关,并与MARBLES中-0.60％（-1.08，-0.13）较低的平均阵列DNA甲基化相关。产前维生素摄入量和单个DNA甲基化位点效应估计在队列和组织之间的关联几乎没有一致性。只有几个重叠的站点具有相关的效果估计。然而,p值<0.01的单个DNA甲基化位点(EARLI脐带nCpGs=4068,EARLI胎盘nCpGs=3647,MARBLES脐带nCpGs=4068,MARBLES胎盘nCpGs=9563)在神经元发育途径中持续富集。
一起,我们的研究结果表明,妊娠首月产前维生素摄入量可能与胎盘整体DNA甲基化水平降低有关,也与胎盘和脐带血大脑相关通路的DNA甲基化有关.
在胎盘中，在EARLI中,产前维生素摄入量与-0.52%(95%CI-1.04,0.01)较低的平均阵列DNA甲基化相关,并与MARBLES中-0.60％（-1.08，-0.13）较低的平均阵列DNA甲基化相关。产前维生素摄入量和单个DNA甲基化位点效应估计在队列和组织之间的关联几乎没有一致性。只有几个重叠的站点具有相关的效果估计。然而,p值<0.01的单个DNA甲基化位点(EARLI脐带nCpGs=4068,EARLI胎盘nCpGs=3647,MARBLES脐带nCpGs=4068,MARBLES胎盘nCpGs=9563)在神经元发育途径中持续富集。
一起,我们的研究结果表明,妊娠首月产前维生素摄入量可能与胎盘整体DNA甲基化水平降低有关,也与胎盘和脐带血大脑相关通路的DNA甲基化有关.
