Abstract：
Little is known about the association between bacterial DNA in human blood and the risk of cardiovascular disease (CVD) mortality.
A case-cohort study was performed based on a 9 ½ year follow-up of the Oslo II study from 2000. Eligible for this analysis were men born in 1923 and from 1926 to 1932. The cases were men (n = 227) who had died from CVD, and the controls were randomly selected participants from the same cohort (n = 178). Analysis of the bacterial microbiome was performed on stored frozen blood samples for both cases and controls. Association analyses for CVD mortality were performed by Cox proportional hazard regression adapted to the case-cohort design. We used the Bonferroni correction due to the many bacterial genera that were identified.
Bacterial DNA was identified in 372 (82%) of the blood samples and included 78 bacterial genera from six phyla. Three genera were significantly associated with CVD mortality. The genera Kocuria (adjusted hazard ratio (HR) 8.50, 95% confidence interval (CI) (4.05, 17.84)) and Enhydrobacter (HR 3.30 (2.01, 5.57)) indicate an association with CVD mortality with increasing levels. The genera Paracoccus (HR 0.29 (0.15, 0.57)) was inversely related. Significant predictors of CVD mortality were: the feeling of bad health; and the consumption of more than three cups of coffee per day. The following registered factors were borderline significant, namely: a history of heart failure; increased systolic blood pressure; and currently taking antihypertensive drugs now, versus previously.
The increasing levels of two bacterial genera Kocuria (skin and oral) and Enhydrobacter (skin) and low levels of Paracoccus (soil) were associated with CVD mortality independent of known risk factors for CVD.
A case-cohort study was performed based on a 9 ½ year follow-up of the Oslo II study from 2000. Eligible for this analysis were men born in 1923 and from 1926 to 1932. The cases were men (n = 227) who had died from CVD, and the controls were randomly selected participants from the same cohort (n = 178). Analysis of the bacterial microbiome was performed on stored frozen blood samples for both cases and controls. Association analyses for CVD mortality were performed by Cox proportional hazard regression adapted to the case-cohort design. We used the Bonferroni correction due to the many bacterial genera that were identified.
Bacterial DNA was identified in 372 (82%) of the blood samples and included 78 bacterial genera from six phyla. Three genera were significantly associated with CVD mortality. The genera Kocuria (adjusted hazard ratio (HR) 8.50, 95% confidence interval (CI) (4.05, 17.84)) and Enhydrobacter (HR 3.30 (2.01, 5.57)) indicate an association with CVD mortality with increasing levels. The genera Paracoccus (HR 0.29 (0.15, 0.57)) was inversely related. Significant predictors of CVD mortality were: the feeling of bad health; and the consumption of more than three cups of coffee per day. The following registered factors were borderline significant, namely: a history of heart failure; increased systolic blood pressure; and currently taking antihypertensive drugs now, versus previously.
The increasing levels of two bacterial genera Kocuria (skin and oral) and Enhydrobacter (skin) and low levels of Paracoccus (soil) were associated with CVD mortality independent of known risk factors for CVD.
摘要：
人类血液中的细菌DNA与心血管疾病（CVD）死亡风险之间的关系知之甚少。
一项病例队列研究是基于2000年对奥斯陆II研究进行的9½年随访。符合此分析的是1923年和1926年至1932年出生的男性。病例为男性（n=227），死于心血管疾病，对照组为来自同一队列的随机选择参与者(n=178).对两种情况和对照的储存的冷冻血液样品进行细菌微生物组的分析。通过适用于病例队列设计的Cox比例风险回归进行CVD死亡率的关联分析。由于鉴定了许多细菌属，我们使用了Bonferroni校正。
在372（82％）的血液样品中鉴定出细菌DNA，其中包括来自6个门的78个细菌属。三个属与CVD死亡率显著相关。Kocuria属（调整后的危险比（HR）8.50，95％置信区间（CI）（4.05，17.84））和Enhydupacter（HR3.30（2.01，5.57））表明与CVD死亡率相关，水平升高。副球菌属（HR0.29（0.15，0.57））呈负相关。心血管疾病死亡率的重要预测因素是：健康状况不佳；每天饮用超过三杯咖啡。以下登记因素为临界显著，即：心力衰竭史；收缩压升高；目前正在服用降压药，与以前相比。
两种细菌属Kocuria（皮肤和口腔）和Enhydrobacter（皮肤）的水平升高以及副球菌（土壤）的水平降低与CVD死亡率相关，而与已知的CVD危险因素无关。
一项病例队列研究是基于2000年对奥斯陆II研究进行的9½年随访。符合此分析的是1923年和1926年至1932年出生的男性。病例为男性（n=227），死于心血管疾病，对照组为来自同一队列的随机选择参与者(n=178).对两种情况和对照的储存的冷冻血液样品进行细菌微生物组的分析。通过适用于病例队列设计的Cox比例风险回归进行CVD死亡率的关联分析。由于鉴定了许多细菌属，我们使用了Bonferroni校正。
在372（82％）的血液样品中鉴定出细菌DNA，其中包括来自6个门的78个细菌属。三个属与CVD死亡率显著相关。Kocuria属（调整后的危险比（HR）8.50，95％置信区间（CI）（4.05，17.84））和Enhydupacter（HR3.30（2.01，5.57））表明与CVD死亡率相关，水平升高。副球菌属（HR0.29（0.15，0.57））呈负相关。心血管疾病死亡率的重要预测因素是：健康状况不佳；每天饮用超过三杯咖啡。以下登记因素为临界显著，即：心力衰竭史；收缩压升高；目前正在服用降压药，与以前相比。
两种细菌属Kocuria（皮肤和口腔）和Enhydrobacter（皮肤）的水平升高以及副球菌（土壤）的水平降低与CVD死亡率相关，而与已知的CVD危险因素无关。
