PDF(Pubmed)
Abstract：
Diet is a major driver of the structure and function of the gut microbiota, which influences the host physiology. Alcohol abuse can induce liver disease and gut microbiota dysbiosis. Here, we aim to elucidate whether the well-known traditional health food Goji berry targets gut microbiota to prevent liver injury induced by acute alcohol intake. The results showed that Goji supplementation for 14 days alleviated acute liver injury as indicated by lowering serum aspartate aminotransferase, alanine aminotransferase, pro-inflammatory cytokines, as well as lipopolysaccharide content in the liver tissue. Goji maintained the integrity of the epithelial barrier and increased the levels of butyric acid in cecum contents. Furthermore, we established the causal relationship between gut microbiota and liver protection effects of Goji with the help of antibiotics treatment and fecal microbiota transplantation (FMT) experiments. Both Goji and FMT-Goji increased glutathione (GSH) in the liver and selectively enriched the butyric acid-producing gut bacterium Akkermansia and Ruminococcaceae by using 16S rRNA gene sequencing. Metabolomics analysis of cecum samples revealed that Goji and its trained microbiota could regulate retinoyl β-glucuronide, vanillic acid, and increase the level of glutamate and pyroglutamic acid, which are involved in GSH metabolism. Our study highlights the communication among Goji, gut microbiota, and liver homeostasis.
摘要：
饮食是肠道微生物群结构和功能的主要驱动因素，影响宿主的生理机能。酗酒可引起肝脏疾病和肠道微生物群失调。这里,我们的目的是阐明著名的传统保健食品枸杞是否针对肠道菌群，以预防急性酒精摄入引起的肝损伤。结果表明，补充枸杞14天可通过降低血清天冬氨酸转氨酶来减轻急性肝损伤，丙氨酸氨基转移酶，促炎细胞因子，以及肝组织中的脂多糖含量。Goji保持了上皮屏障的完整性，并增加了盲肠内容物中丁酸的水平。此外，我们借助抗生素治疗和粪便菌群移植（FMT）实验，建立了肠道菌群与枸杞肝脏保护作用之间的因果关系。Goji和FMT-Goji都增加了肝脏中的谷胱甘肽（GSH），并通过使用16SrRNA基因测序选择性富集了产生丁酸的肠道细菌Akkermansia和Ruminoccaceae。盲肠样品的代谢组学分析显示，枸杞及其训练的微生物群可以调节视黄酰β-葡糖苷酸，香草酸,并增加谷氨酸和焦谷氨酸的水平，参与GSH代谢。我们的研究强调了Goji之间的交流，肠道菌群，和肝脏稳态。
