Abstract：
Goatpox, sheeppox, and peste-des-petits-ruminants (PPR) are economically important virus diseases affecting goats and sheep, which often cause coinfection/comorbidities in the field. Coinfection with these viruses leads to enhanced infection in natural scenarios in terms of morbidities and mortalities. Currently, individual live attenuated vaccines are being used to mitigate these diseases and research on combination vaccines for these diseases is encouraging. For the preparation of combination vaccines, vaccine strains of the peste-des-petits-ruminants virus (PPRV), goatpox virus (GTPV), and sheeppox virus (SPPV) are grown separately and GTPV + PPRV are mixed for vaccination of goats, and PPRV + SPPV for sheep. Growing capripox and PPRV strains in the same cells simultaneously without the titer loss will save the time and cost of production. In the current study, we have evaluated the coinfection kinetics of capripox virus and a PPRV using a candidate GTPV vaccine strain (originally caused infection in both goats and sheep in the field) and PPRV/Sungri/96 (vaccine strain) in Vero cells. At high multiplicity of infection (MOI), PPRV was excluded from coinfection by GTPV, whereas at a low multiplicity coexistence/accommodation was observed between PPRV and GTPV without loss of the titer. The results shed light on the possibility of the production of two vaccine strains in the same cells using the coinfection model economically.
摘要：
山痘,羊痘,和小反刍动物（PPR）是影响山羊和绵羊的经济上重要的病毒病，这通常会导致该领域的共感染/合并症。与这些病毒共感染导致在自然情况下在发病率和死亡率方面增强的感染。目前,个体减毒活疫苗正被用于缓解这些疾病，对这些疾病的联合疫苗的研究令人鼓舞。为了制备联合疫苗，peste-des-pitts-反刍动物病毒（PPRV）的疫苗株，山羊痘病毒（GTPV），和羊痘病毒（SPPV）分别生长，GTPV+PPRV混合接种山羊，和绵羊的PPRV+SPPV。在没有滴度损失的情况下,在相同细胞中同时生长卷羊痘和PPRV菌株将节省生产的时间和成本。在目前的研究中,我们使用候选GTPV疫苗株(最初在田间山羊和绵羊中引起感染)和Vero细胞中的PPRV/Sungri/96(疫苗株)评估了羊痘病毒和PPRV的共感染动力学.在高感染复数（MOI），PPRV被GTPV排除在合并感染之外，而在低多重性下，PPRV和GTPV之间观察到共存/调节，而没有滴度的损失。结果揭示了使用共感染模型在相同细胞中经济地生产两种疫苗菌株的可能性。
