PDF(Pubmed)
Abstract：
Aeromonas dhakensis is ubiquitous in aquatic habitats and can cause life-threatening septicaemia in humans. However, limited data are available on their antimicrobial susceptibility testing (AST) profiles. Hence, we aimed to examine their AST patterns using clinical (n = 94) and non-clinical (n = 23) isolates with dehydrated MicroScan microdilution. Carbapenem resistant isolates were further screened for genes related to carbapenem resistance using molecular assay. The isolates exhibited resistance to imipenem (76.9%), doripenem (62.4%), meropenem (41.9%), trimethoprim/sulfamethoxazole (11.1%), cefotaxime (8.5%), ceftazidime (6%), cefepime (1.7%) and aztreonam (0.9%), whereas all isolates were susceptible to amikacin. Clinical isolates showed significant association with resistance to doripenem, imipenem and meropenem compared to non-clinical isolates. These blacphA were detected in clinical isolates with resistance phenotypes: doripenem (67.2%, 45/67), imipenem (65.9%, 54/82) and meropenem (65.2%, 30/46). Our findings showed that the MicroScan microdilution method is suitable for the detection of carbapenem resistance in both clinical (48.9-87.2%) and non-clinical (4.3-13.0%) isolates. This study revealed that A. dhakensis isolates had relatively high carbapenem resistance, which may lead to potential treatment failure. Continued monitoring of aquatic sources with a larger sample size should be carried out to provide further insights.
摘要：
dhakensis气单胞菌在水生生境中无处不在，可引起危及生命的败血症。然而,关于抗菌药物敏感性试验(AST)的数据有限.因此，我们旨在使用脱水MicroScan微量稀释的临床（n=94）和非临床（n=23）分离株检查其AST模式。采用分子检测方法进一步筛选碳青霉烯类耐药分离株中与碳青霉烯类耐药相关的基因。分离株对亚胺培南耐药率(76.9%),多利培南(62.4%),美罗培南（41.9%），甲氧苄啶/磺胺甲恶唑（11.1%），头孢噻肟（8.5%），头孢他啶(6%),头孢吡肟(1.7%)和氨曲南(0.9%)，而所有分离株均对阿米卡星敏感。临床分离株显示与多尼培南耐药性显著相关，亚胺培南和美罗培南与非临床分离株相比。在具有抗性表型的临床分离株中检测到这些blacphA：多尼培南（67.2％，45/67),亚胺培南（65.9%，54/82)和美罗培南(65.2%，30/46).我们的发现表明，MicroScan微量稀释方法适用于临床（48.9-87.2％）和非临床（4.3-13.0％）分离株中碳青霉烯耐药性的检测。这项研究表明，达克氏菌分离株具有相对较高的碳青霉烯抗性，这可能导致潜在的治疗失败。应继续监测样本量较大的水生源，以提供进一步的见解。
