Abstract：
Herbicides are the most commonly used means of controlling the growth of Ambrosia artemisiifolia L. Their constant use has led to the development of resistant populations. They can be evaluated by studying seed germination and the corresponding grown plants, but A. artemisiifolia exhibits seed dormancy, preventing germination and delaying research. Here, we developed a simple and rapid method to stimulate germination of freshly collected or stored A. artemisiifolia seeds. The germination of A. artemisiifolia freshly collected/stored seeds was evaluated after storage, stratification, and chemical treatments (ethephon, gibberellic acid (GA3), thiourea, KNO3). Ethephon or ethephon + GA3 improved freshly collected seed germination by 88 and 95%, respectively, and germination of stored seeds by 78 and 80%, respectively. In addition, placing the seeds of A. artemisiifolia in ethephon, GA3, ethephon + GA3, or thiourea solutions caused the freshly collected seeds to germinate faster than stored seeds or nontreated seeds. In contrast, the conditioning of seeds in these solutions favored germination of stored seeds, especially when ethephon + GA3 or GA3 was used. Imbibition of the freshly collected A. artemisiifolia seeds in a mixture of ethephon and GA3 can effectively overcome primary dormancy when rapid experimental results are needed. For seeds requiring prolonged storage, conditioning in ethephon, GA3, or thiourea solutions may be applied to promote germination.
摘要：
除草剂是控制青蒿生长的最常用手段。它们的不断使用导致了抗性种群的发展。可以通过研究种子发芽和相应的生长植物来评估它们，但是青蒿表现出种子休眠，防止发芽和延缓研究。这里,我们开发了一种简单快速的方法来刺激新鲜收集或储存的青蒿种子发芽。储存后评估新鲜收集/储存的青蒿种子的萌发，分层，和化学处理(乙烯利，赤霉素（GA3），硫脲,KNO3).乙烯利或乙烯利+GA3使新鲜收集的种子发芽率提高了88％和95％，分别,储存的种子发芽了78%和80%，分别。此外,将青蒿属的种子放在乙烯利中，GA3，乙烯利GA3或硫脲溶液使新鲜收集的种子比储存的种子或未处理的种子发芽更快。相比之下,这些溶液中种子的调理有利于储存种子的发芽，特别是当使用乙烯利+GA3或GA3时。当需要快速的实验结果时，在乙烯利和GA3的混合物中吸收新鲜收集的青蒿种子可以有效地克服初级休眠。对于需要长期储存的种子，在乙烯利中调理，可以应用GA3或硫脲溶液以促进发芽。
