Abstract：
Cell regenerative therapy is a modern solution for difficult-to-heal wounds. Keratinocytes, the most common cell type in the skin, are difficult to obtain without the creation of another wound. Stem cell differentiation towards keratinocytes is a challenging process, and it is difficult to reproduce in chemically defined media. Nevertheless, a co-culture of keratinocytes with stem cells usually achieves efficient differentiation. This systematic review aims to identify the secretions of normal human keratinocytes reported in the literature and correlate them with the differentiation process. An online search revealed 338 references, of which 100 met the selection criteria. A total of 80 different keratinocyte secretions were reported, which can be grouped mainly into cytokines, growth factors, and antimicrobial peptides. The growth-factor group mostly affects stem cell differentiation into keratinocytes, especially epidermal growth factor and members of the transforming growth factor family. Nevertheless, the reported secretions reflected the nature of the involved studies, as most of them focused on keratinocyte interaction with inflammation. This review highlights the secretory function of keratinocytes, as well as the need for intense investigation to characterize these secretions and evaluate their regenerative capacities.
摘要：
细胞再生疗法是用于难以愈合的伤口的现代解决方案。角质形成细胞,皮肤中最常见的细胞类型，在没有另一个伤口的情况下很难获得。干细胞向角质形成细胞分化是一个具有挑战性的过程，并且很难在化学成分确定的培养基中复制。然而，角质形成细胞与干细胞的共培养通常实现有效分化。本系统综述旨在鉴定文献中报道的正常人角质形成细胞的分泌物，并将其与分化过程相关联。网上搜索发现了338个参考资料，其中100人符合选择标准。总共报告了80种不同的角质形成细胞分泌物，可以主要分为细胞因子，生长因子，和抗菌肽。生长因子组主要影响干细胞分化为角质形成细胞，特别是表皮生长因子和转化生长因子家族的成员。然而，报告的分泌物反映了所涉及研究的性质，因为他们大多集中在角质形成细胞与炎症的相互作用。这篇综述强调了角质形成细胞的分泌功能，以及需要进行激烈的调查来表征这些分泌物并评估其再生能力。
