Abstract：
The widespread escalation of bacterial resistance threatens the safety of the food chain. To investigate the resistance characteristics of E. coli strains isolated from disinfected tableware against both disinfectants and antibiotics, 311 disinfected tableware samples, including 54 chopsticks, 32 dinner plates, 61 bowls, 11 cups, and three spoons were collected in Chengdu, Sichuan Province, China to screen for disinfectant- (benzalkonium chloride and cetylpyridinium chloride) and tigecycline-resistant isolates, which were then subjected to antimicrobial susceptibility testing and whole genome sequencing (WGS). The coliform-positive detection rate was 51.8% (161/311) and among 161 coliform-positive samples, eight E. coli strains were multidrug-resistant to benzalkonium chloride, cetylpyridinium chloride, ampicillin, and tigecycline. Notably, a recently described mobile colistin resistance gene mcr-10 present on the novel IncFIB-type plasmid of E. coli EC2641 screened was able to successfully transform the resistance. Global phylogenetic analysis revealed E. coli EC2641 clustered together with two clinically disinfectant- and colistin-multidrug-resistant E. coli strains from the US. This is the first report of mcr-10-bearing E. coli detected in disinfected tableware, suggesting that continuous monitoring of resistance genes in the catering industry is essential to understand and respond to the transmission of antibiotic resistance genes from the environment and food to humans and clinics.
摘要：
细菌耐药性的广泛升级威胁着食物链的安全。调查从消毒餐具中分离的大肠杆菌对消毒剂和抗生素的耐药性特征。311个消毒餐具样品，包括54根筷子,32个餐盘，61碗，11杯，在成都收集了三个勺子，四川省,中国将筛选消毒剂（苯扎氯铵和氯化十六烷基吡啶）和替加环素抗性分离株，然后对其进行抗菌药物敏感性测试和全基因组测序（WGS）。大肠杆菌阳性检出率为51.8%(161/311),在161份大肠杆菌阳性样本中,八株大肠杆菌对苯扎氯铵具有多重耐药性，氯化十六烷基吡啶,氨苄青霉素,还有替加环素.值得注意的是,筛选的大肠杆菌EC2641的新型IncFIB型质粒上最近描述的可移动粘菌素抗性基因mcr-10能够成功转化抗性。全球系统发育分析显示，大肠杆菌EC2641与来自美国的两种临床消毒剂和粘菌素多重耐药大肠杆菌菌株聚集在一起。这是首次在消毒餐具中检测到含mcr-10大肠杆菌的报告，这表明，持续监测餐饮业的抗性基因对于了解和应对抗生素抗性基因从环境和食物向人类和诊所的传播至关重要。
