Abstract：
We previously reported that IgA vasculitis and cutaneous arteritis could be dependently associated with the presence of the antiphosphatidylserine/prothrombin complex (anti-PS/PT) antibody and lysosomal-associated membrane protein-2 (LAMP-2). The copy number of LAMP-2 mRNA in skin tissue samples from rats with cutaneous vasculitis induced by intravenous administration of anti-PS/PT antibody after subcutaneous histone injection was significantly higher than in those without cutaneous vasculitis. We found LAMP-2 protein overexpression in neutrophils and vascular endothelial cells of the affected blood vessels in rats with cutaneous vasculitis induced by intravenous administration of anti-PS/PT antibody after cutaneous priming by histones. We found typical cutaneous small-vessel vasculitis in the skin of rats given intravenous injection of both anti-PS/PT antibody and anti-LAMP-2 antibody after cutaneous priming by histones. We suggested that the introduction of skin local histones and anti-PS/PT antibody in serum could move LAMP-2 to the cell surface of neutrophils and vascular endothelial cells, and that anti-LAMP-2 antibody could bridge these cells through antigen-specific binding in typical cutaneous small-vessel vasculitis.
摘要：
我们先前报道了IgA血管炎和皮肤动脉炎可能与抗磷脂酰丝氨酸/凝血酶原复合物（抗PS/PT）抗体和溶酶体相关膜蛋白2（LAMP-2）的存在相关。皮下组蛋白注射后静脉内给予抗PS/PT抗体诱导的皮肤血管炎大鼠皮肤组织样本中LAMP-2mRNA的拷贝数明显高于无皮肤血管炎大鼠。我们发现，在组蛋白引发皮肤后，静脉内给予抗PS/PT抗体诱导的皮肤血管炎大鼠中，受影响血管的中性粒细胞和血管内皮细胞中LAMP-2蛋白过表达。我们在组蛋白皮肤引发后静脉注射抗PS/PT抗体和抗LAMP-2抗体的大鼠皮肤中发现了典型的皮肤小血管血管炎。我们建议在血清中引入皮肤局部组蛋白和抗PS/PT抗体可以将LAMP-2移动到中性粒细胞和血管内皮细胞的细胞表面，在典型的皮肤小血管血管炎中，抗LAMP-2抗体可以通过抗原特异性结合来桥接这些细胞。
