PDF(Pubmed)
Abstract：
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes the coronavirus disease 2019 (COVID-19), ranging from asymptomatic conditions to severe/fatal lung injury and multi-organ failure. Growing evidence shows that the nasopharyngeal microbiota composition may predict the severity of respiratory infections and may play a role in the protection from viral entry and the regulation of the immune response to the infection. In the present study, we have characterized the nasopharyngeal bacterial microbiota (BNM) composition and have performed factor analysis in a group of 54 asymptomatic/paucisymptomatic subjects who tested positive for nasopharyngeal swab SARS-CoV-2 RNA and/or showed anti-RBD-IgG positive serology at the enrolment. We investigated whether BNM was associated with SARS-CoV-2 RNA positivity and serum anti-RBD-IgG antibody development/maintenance 20-28 weeks after the enrolment. Shannon\'s entropy α-diversity index [odds ratio (OR) = 5.75, p = 0.0107] and the BNM Factor1 (OR = 2.64, p = 0.0370) were positively associated with serum anti-RBD-IgG antibody maintenance. The present results suggest that BNM composition may influence the immunological memory against SARS-CoV-2 infections. To the best of our knowledge, this is the first study investigating the link between BNM and specific IgG antibody maintenance. Further studies are needed to unveil the mechanisms through which the BNM influences the adaptive immune response against viral infections.
摘要：
严重急性呼吸道综合症冠状病毒2（SARS-CoV-2）导致2019年冠状病毒病（COVID-19），从无症状到严重/致命的肺损伤和多器官衰竭。越来越多的证据表明，鼻咽微生物群组成可以预测呼吸道感染的严重程度，并可能在保护病毒进入和调节对感染的免疫反应中发挥作用。在本研究中,我们对鼻咽细菌微生物群(BNM)组成进行了表征,并对54例无症状/无症状受试者进行了因子分析,这些受试者在入选时鼻咽拭子SARS-CoV-2RNA检测呈阳性和/或抗RBD-IgG血清学检测呈阳性.我们调查了BNM是否与SARS-CoV-2RNA阳性和血清抗RBD-IgG抗体开发/维持相关20-28周。香农熵α-多样性指数[比值比（OR）=5.75，p=0.0107]和BNM因子1（OR=2.64，p=0.0370）与血清抗RBD-IgG抗体维持呈正相关。目前的结果表明，BNM成分可能会影响针对SARS-CoV-2感染的免疫记忆。据我们所知,这是第一项调查BNM与特异性IgG抗体维持之间联系的研究.需要进一步的研究来揭示BNM影响针对病毒感染的适应性免疫应答的机制。
