PDF(Pubmed)
Abstract：
Obesity has been a growing problem in young patients leading to serious metabolic complications. There are many studies supporting the idea, that obesity should be considered as a chronic inflammation closely associated with immune system alterations. Th17 subpopulation is strongly involved in this process. The aim of our study was to evaluate circulating Th17 cells in overweight and obese children and explore the relationships between Th17 subset and metabolic parameters.
We evaluated peripheral Th17 cells in fresh peripheral blood samples from 27 overweight and obese and 15 normal-weight children. Th17 cells were identified by flow cytometry using monoclonal antibody and intracellular IL-17A staining. Th17 cells were defined as CD3+CD4+CD196+IL-17Aic+. The analysis involved anthropometric and metabolic parameters measured at baseline and three months after the change of lifestyle and diet. We evaluated the relationship between metabolic parameters and Th17 cells.
In overweight and obese children we found significantly higher Th17 cells percentage compared to normal weight controls (median 0.097% (0.044 - 0.289) vs 0.041% (0.023 - 0.099), p = 0.048). The percentage of Th17 cells decreased statistically significantly in children who reduced weight after the intervention (0.210% (0.143 - 0.315) vs 0.039% (0.028 - 0.106), p = 0.004). In this group we also noticed statistically significant reduction of TC and LDL-C concentration (p = 0.01, p = 0.04, respectively).
Obesity in children is associated with increased percentage of peripheral Th17 cells. Weight reduction leads to significant decrease of circulating Th17 cells and improvement of lipid parameters. This significant reduction of proinflammatory Th17 cells is a promising finding suggesting that obesity-induced inflammation in children could be relatively easily reversible.
We evaluated peripheral Th17 cells in fresh peripheral blood samples from 27 overweight and obese and 15 normal-weight children. Th17 cells were identified by flow cytometry using monoclonal antibody and intracellular IL-17A staining. Th17 cells were defined as CD3+CD4+CD196+IL-17Aic+. The analysis involved anthropometric and metabolic parameters measured at baseline and three months after the change of lifestyle and diet. We evaluated the relationship between metabolic parameters and Th17 cells.
In overweight and obese children we found significantly higher Th17 cells percentage compared to normal weight controls (median 0.097% (0.044 - 0.289) vs 0.041% (0.023 - 0.099), p = 0.048). The percentage of Th17 cells decreased statistically significantly in children who reduced weight after the intervention (0.210% (0.143 - 0.315) vs 0.039% (0.028 - 0.106), p = 0.004). In this group we also noticed statistically significant reduction of TC and LDL-C concentration (p = 0.01, p = 0.04, respectively).
Obesity in children is associated with increased percentage of peripheral Th17 cells. Weight reduction leads to significant decrease of circulating Th17 cells and improvement of lipid parameters. This significant reduction of proinflammatory Th17 cells is a promising finding suggesting that obesity-induced inflammation in children could be relatively easily reversible.
摘要：
肥胖已成为年轻患者中日益严重的问题，导致严重的代谢并发症。有许多研究支持这个想法，肥胖应被视为与免疫系统改变密切相关的慢性炎症。Th17亚群强烈参与这一过程。我们研究的目的是评估超重和肥胖儿童的循环Th17细胞，并探讨Th17亚群与代谢参数之间的关系。
我们评估了27名超重和肥胖儿童和15名正常体重儿童的新鲜外周血样本中的外周血Th17细胞。使用单克隆抗体和细胞内IL-17A染色通过流式细胞术鉴定Th17细胞。Th17细胞定义为CD3+CD4+CD196+IL-17Aic+。该分析涉及在基线和生活方式和饮食改变后三个月测量的人体测量和代谢参数。我们评估了代谢参数与Th17细胞之间的关系。
在超重和肥胖儿童中，我们发现Th17细胞百分比明显高于正常体重对照组（中位数0.097％（0.044-0.289）对0.041％（0.023-0.099），p=0.048)。Th17细胞的百分比在干预后体重下降的儿童中具有统计学意义（0.210％（0.143-0.315）对0.039％（0.028-0.106），p=0.004)。在该组中，我们还注意到TC和LDL-C浓度的统计学显着降低（分别为p=0.01，p=0.04）。
儿童肥胖与外周Th17细胞百分比增加有关。体重减轻导致循环Th17细胞的显著减少和脂质参数的改善。促炎性Th17细胞的这种显着减少是一个有希望的发现，表明肥胖引起的儿童炎症可能相对容易逆转。
我们评估了27名超重和肥胖儿童和15名正常体重儿童的新鲜外周血样本中的外周血Th17细胞。使用单克隆抗体和细胞内IL-17A染色通过流式细胞术鉴定Th17细胞。Th17细胞定义为CD3+CD4+CD196+IL-17Aic+。该分析涉及在基线和生活方式和饮食改变后三个月测量的人体测量和代谢参数。我们评估了代谢参数与Th17细胞之间的关系。
在超重和肥胖儿童中，我们发现Th17细胞百分比明显高于正常体重对照组（中位数0.097％（0.044-0.289）对0.041％（0.023-0.099），p=0.048)。Th17细胞的百分比在干预后体重下降的儿童中具有统计学意义（0.210％（0.143-0.315）对0.039％（0.028-0.106），p=0.004)。在该组中，我们还注意到TC和LDL-C浓度的统计学显着降低（分别为p=0.01，p=0.04）。
儿童肥胖与外周Th17细胞百分比增加有关。体重减轻导致循环Th17细胞的显著减少和脂质参数的改善。促炎性Th17细胞的这种显着减少是一个有希望的发现，表明肥胖引起的儿童炎症可能相对容易逆转。
