PDF(Pubmed)
Abstract：
Enzymes have essential roles in catalyzing biological reactions and maintaining metabolic systems. Many in vitro enzymatic bioassays have been developed for use in industrial and research fields, such as cell biology, enzyme engineering, drug screening, and biofuel production. Of note, many of these require the use of high-throughput platforms. Although the microtiter plate remains the standard for high-throughput enzymatic bioassays, microfluidic arrays and droplet microfluidics represent emerging methods. Each has seen significant advances and offers distinct advantages; however, drawbacks in key performance metrics, including reagent consumption, reaction manipulation, reaction recovery, real-time measurement, concentration gradient range, and multiplexity, remain. Herein, we compare recent high-throughput platforms using the aforementioned metrics as criteria and provide insights into remaining challenges and future research trends.
摘要：
酶在催化生物反应和维持代谢系统中具有重要作用。许多体外酶生物测定法已经被开发用于工业和研究领域，比如细胞生物学，酶工程,药物筛选,和生物燃料生产。值得注意的是，其中许多需要使用高通量平台。尽管微量滴定板仍然是高通量酶促生物测定的标准，微流体阵列和液滴微流体代表新兴的方法。每个人都取得了重大进展，并提供了独特的优势；然而，关键性能指标的缺点，包括试剂消耗，反应操纵，反应恢复,实时测量,浓度梯度范围,和多重性，remain.在这里，我们使用上述指标作为标准比较了最近的高通量平台，并提供了对剩余挑战和未来研究趋势的见解。
