PDF(Pubmed)
Abstract：
Oral microbiome sequencing efforts revealed the presence of hundreds of different microbes. Interindividual differences at strain and species resolution suggest that microbiome diversity could lead to mechanistically distinct gene regulation as well as species-related differences in phenotypes. Commonly, gene regulation and related phenotypes are studied in a few selected strains of a particular species with conclusions that are mostly generalized. The aim of this study was to isolate several species of Corynebacterium using an established protocol that led to the previous isolation of C. durum. Characterization of C. durum interspecies interactions revealed a specific mechanism for chain elongation in Streptococcus sanguinis that was the result of corynebacterial fatty acid production and secretion. While the protocol was successfully applied to isolate what we presumed to be additional Corynebacterium based on several phenotypic traits that seem to be identical to C. durum, genome sequencing of the newly isolated strains placed them closer to Actinomyces. Both Corynebacterium and Actinomyces are suborders of the Actinobacteridae and related species. Our study suggests to take several comprehensive strategies into consideration when taxonomically identifying closely related microorganisms. Furthermore, it seems to be important to test common core phenotypes in bacterial ecology to understand the behavior of specific groups of microbes, rather than simply relying upon genome sequence homology to establish relationships in the microbiome.
摘要：
口腔微生物组测序工作揭示了数百种不同微生物的存在。菌株和物种分辨率的个体差异表明，微生物组多样性可能导致机制上不同的基因调控以及物种相关的表型差异。通常,在特定物种的一些选定菌株中研究了基因调控和相关表型，得出的结论大多是通用的。这项研究的目的是使用已建立的方案分离几种棒状杆菌，该方案导致先前分离硬粒C.硬粒梭菌种间相互作用的表征揭示了血链球菌链延长的特定机制，这是棒状杆菌脂肪酸产生和分泌的结果。虽然该方案被成功地应用于分离我们认为是额外的棒状杆菌，基于几个表型性状，似乎是相同的硬粒。新分离的菌株的基因组测序使它们更接近放线菌。棒状杆菌和放线菌都是放线菌科及相关物种的亚目。我们的研究建议在分类识别密切相关的微生物时考虑几种综合策略。此外，在细菌生态学中测试常见的核心表型以了解特定微生物群的行为似乎很重要，而不是简单地依靠基因组序列同源性来建立微生物组中的关系。
