PDF(Pubmed)
Abstract：
Bone disease and disorder treatment might be difficult because of its complicated nature. Millions of patients each year need bone substitutes that may help them recover quickly from a variety of illnesses. Synthetic bone replacements that mirror the structural, chemical, and biological features of bone matrix structure will be very helpful and in high demand. In this research, the inorganic bioactive glass nanoparticles matrixed with organic collagen and silk fibroin structure (COL/SF/CaO-SiO2) were used to create multifunctional bone-like fibers in this study, which we describe here. The fiber structure is organized in a layered fashion comparable to the sequence in which apatite and neo tissue are formed. The amino groups in COL and SF combined with CaO-SiO2 to stabilize the resulting composite nanofiber. Morphological and functional studies confirmed that crystalline CaO-SiO2 nanoparticles with average sizes of 20 ± 5 nm are anchored on a 115 ± 10 nm COL/SF nanofiber matrix. X-ray photoelectron spectroscopic (XPS) results confirmed the presence of C, N, O, Ca, and Si in the composite fiber with an atomic percentage of 59.46, 3.30, 20.25, 3.38 and 13.61%. respectively. The biocompatibility examination with osteoblast cells (Saos-2) revealed that the CAL/SF/CaO-SiO2 composite nanofiber had enhanced osteogenic activity. Finally, when the CAL/SF/CaO-SiO2 composite nanofiber scaffolds were used to treat an osteoporotic bone defect in a rat model, the composite nanofiber scaffolds significantly promoted bone regeneration and vascularization. This novel fibrous scaffold class represents a potential breakthrough in the design of advanced materials for complicated bone regeneration.
摘要：
骨疾病和疾病的治疗可能是困难的，因为它的复杂性。每年有数百万患者需要骨骼替代品，这可能有助于他们从各种疾病中迅速康复。反映结构的合成骨替代物，化学,和骨基质结构的生物学特征将非常有帮助和高要求。在这项研究中,在这项研究中，用有机胶原蛋白和丝素蛋白结构（COL/SF/CaO-SiO2）基质的无机生物活性玻璃纳米颗粒用于创建多功能骨样纤维，我们在这里描述。纤维结构以与形成磷灰石和新组织的顺序相当的分层方式组织。COL和SF中的氨基与CaO-SiO2结合以稳定所得复合纳米纤维。形态和功能研究证实，平均尺寸为20±5nm的结晶CaO-SiO2纳米颗粒锚定在115±10nm的COL/SF纳米纤维基质上。X射线光电子能谱(XPS)结果证实了C,N,O,Ca,和Si在复合纤维中的原子百分比分别为59.46、3.30、20.25、3.38和13.61%。分别。与成骨细胞（Saos-2）的生物相容性检查表明，CAL/SF/CaO-SiO2复合纳米纤维具有增强的成骨活性。最后,CAL/SF/CaO-SiO2复合纳米纤维支架用于治疗大鼠骨质疏松性骨缺损,复合纳米纤维支架显著促进骨再生和血管化。这种新型纤维支架类别代表了用于复杂骨再生的先进材料设计的潜在突破。
