Abstract：
The present protocol describes a bioluminescence reporter assay developed to quantify the ability of synthetic agonists of retinoic acid receptors (RARs) to activate glutamate receptor subunit 1 (GluR1) translation. The reporter assay uses firefly luciferase under the control of the GluR1 5\' untranslated region (5\' UTR) which is bound by RARs to regulate its translation. This method is used to demonstrate the role of RARα in retinoic acid regulation of GluR1 translation. This method may also be used to screen drugs that influence RAR induction of GluR1 translation as an important mechanism controlling learning and memory in the brain.
摘要：
本方案描述了开发用于量化视黄酸受体（RAR）的合成激动剂激活谷氨酸受体亚基1（GluR1）翻译的能力的生物发光报告测定。报告测定使用在GluR15'非翻译区（5'UTR）控制下的萤火虫荧光素酶，该区域被RAR结合以调节其翻译。该方法用于证明RARα在GluR1翻译的视黄酸调节中的作用。该方法还可用于筛选影响GluR1翻译的RAR诱导的药物，这是控制大脑学习和记忆的重要机制。
