Abstract：
Antimicrobial-resistant is a major challenge in to treat infected wounds, and new formulations should be produced. Citral (Citl), chitosan (Chsn), and zinc oxide (ZnO) nanoparticles may accelerate the wound healing process in terms of their antibacterial properties. This new study aimed to investigate the effects of ointments produced from ZnO/Chsn/Citl nanoparticles (NPs) to treat the infected wounds. Following the preparation of ZnO/Chsn/Citl-NPs, swelling behavior, the release of citral, toxicity, and antibacterial properties were evaluated. Base ointment, mupirocin, and ointments made from Chsn-NPs, Chsn/Citl-NPs, and ZnO/Chsn/Citl-NPs were used to treat the mice. The ointments\' effects on wound contraction, total bacterial count, and immunofluorescence staining for TNF-α, TGF-β, and bFGF were tested. The synthesis of ZnO/Chsn/Citl-NPs was validated by XRD, FT-IR, DLS, and TEM findings. In higher dilutions, chitosan/citral and ZnO/Chsn/Citl-NPs indicated better antibacterial activity. Nanoparticles were safe up to concentration of the 0.5 mg/mL. The mice in Chsn/Citl and ZnO/Chsn/Citl-NPs treated groups showed higher (P < 0.05) wound contraction ratio and expressions for bFGF, and lower total bacterial count and expressions for TGF-β and TNF-α compared to control mice. Ointments prepared from ZnO/Chsn/Citl-NPs could compete with the commercial ointment of mupirocin and can be used to treat infected wounds after clinical studies.
摘要：
抗菌素耐药性是治疗感染伤口的主要挑战，并且应该生产新的配方。柠檬醛（Citl），壳聚糖（Chsn），和氧化锌（ZnO）纳米颗粒在其抗菌性能方面可以加速伤口愈合过程。这项新研究旨在研究由ZnO/Chsn/Citl纳米颗粒（NPs）产生的软膏治疗感染伤口的效果。在制备ZnO/Chsn/Citl-NP之后，溶胀行为，柠檬醛的释放，毒性,和抗菌性能进行了评价。基础软膏，莫匹罗星,和由Chsn-NP制成的软膏，Chsn/Citl-NP，和ZnO/Chsn/Citl-NP用于治疗小鼠。药膏对伤口收缩的影响，细菌总数,和免疫荧光染色TNF-α，TGF-β,和bFGF进行测试。通过XRD对ZnO/Chsn/Citl-NPs的合成进行了验证,FT-IR,DLS,和TEM发现。在较高的稀释度，壳聚糖/柠檬醛和ZnO/Chsn/Citl-NPs具有较好的抗菌活性。纳米颗粒在0.5mg/mL的浓度下是安全的。Chsn/Citl和ZnO/Chsn/Citl-NPs处理组小鼠显示出较高(P<0.05)的创伤收缩率和bFGF表达,与对照小鼠相比，细菌总数和TGF-β和TNF-α的表达较低。由ZnO/Chsn/Citl-NP制备的软膏可以与莫匹罗星的商业软膏竞争，并且可以在临床研究后用于治疗感染的伤口。
