UNASSIGNED: A bilateral common carotid artery ligation was used to establish a chronic CIRI model in mice. HT22 cells were treated with Rg1 after OGD/R to study its effect on [Ca2+]i. The open-field test and pole-climbing experiment were used to detect behavioral injury. The laser speckle blood flowmeter was used to measure brain blood flow. The Nissl and H&E staining were used to examine the neuronal damage. The Western blotting was used to examine MAP2, PSD95, Tau, p-Tau, NOX2, PLC, p-PLC, CN, NFAT1, and NLRP1 expression. Calcium imaging was used to test the level of [Ca2+]i.
UNASSIGNED: Rg1 treatment significantly improved cerebral blood flow, locomotion, and limb coordination, reduced ROS production, increased MAP2 and PSD95 expression, and decreased p-Tau, NOX2, p-PLC, CN, NFAT1, and NLRP1 expression. Calcium imaging results showed that Rg1 could inhibit calcium overload and resist the imbalance of calcium homeostasis after OGD/R in HT22 cells.
UNASSIGNED: Rg1 plays a neuroprotective role in attenuating CIRI by inhibiting oxidative stress, calcium overload, and neuroinflammation.
■双侧颈总动脉结扎用于建立小鼠慢性CIRI模型。在OGD/R后用Rg1处理HT22细胞以研究其对[Ca2]i的影响。采用开场试验和爬杆试验检测行为损伤。激光散斑血流流量计用于测量脑血流量。Nissl和H&E染色用于检查神经元损伤。Western印迹用于检查MAP2,PSD95,Tau,p-Tau,NOX2,PLC,p-PLC,CN,NFAT1和NLRP1表达。钙成像用于测试[Ca2]i的水平。
■Rg1治疗可显着改善脑血流量,运动,和肢体协调,减少ROS产生,MAP2和PSD95表达增加,减少p-Tau,NOX2,p-PLC,CN,NFAT1和NLRP1表达。钙成像结果显示,Rg1可抑制HT22细胞OGD/R后钙超载,抵抗钙稳态失衡。
■Rg1通过抑制氧化应激在减轻CIRI中发挥神经保护作用,钙超载,和神经炎症。