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Abstract：
UNASSIGNED: The incidence of ischemic cerebrovascular disease is increasing in recent years and has been one of the leading causes of neurological dysfunction and death. Ginsenoside Rg1 has been found to protect against neuronal damage in many neurodegenerative diseases. However, the effect and mechanism by which Rg1 protects against cerebral ischemia-reperfusion injury (CIRI) are not fully understood. Here, we report the neuroprotective effects of Rg1 treatment on CIRI and its possible mechanisms in mice.
UNASSIGNED: A bilateral common carotid artery ligation was used to establish a chronic CIRI model in mice. HT22 cells were treated with Rg1 after OGD/R to study its effect on [Ca2+]i. The open-field test and pole-climbing experiment were used to detect behavioral injury. The laser speckle blood flowmeter was used to measure brain blood flow. The Nissl and H&E staining were used to examine the neuronal damage. The Western blotting was used to examine MAP2, PSD95, Tau, p-Tau, NOX2, PLC, p-PLC, CN, NFAT1, and NLRP1 expression. Calcium imaging was used to test the level of [Ca2+]i.
UNASSIGNED: Rg1 treatment significantly improved cerebral blood flow, locomotion, and limb coordination, reduced ROS production, increased MAP2 and PSD95 expression, and decreased p-Tau, NOX2, p-PLC, CN, NFAT1, and NLRP1 expression. Calcium imaging results showed that Rg1 could inhibit calcium overload and resist the imbalance of calcium homeostasis after OGD/R in HT22 cells.
UNASSIGNED: Rg1 plays a neuroprotective role in attenuating CIRI by inhibiting oxidative stress, calcium overload, and neuroinflammation.
UNASSIGNED: A bilateral common carotid artery ligation was used to establish a chronic CIRI model in mice. HT22 cells were treated with Rg1 after OGD/R to study its effect on [Ca2+]i. The open-field test and pole-climbing experiment were used to detect behavioral injury. The laser speckle blood flowmeter was used to measure brain blood flow. The Nissl and H&E staining were used to examine the neuronal damage. The Western blotting was used to examine MAP2, PSD95, Tau, p-Tau, NOX2, PLC, p-PLC, CN, NFAT1, and NLRP1 expression. Calcium imaging was used to test the level of [Ca2+]i.
UNASSIGNED: Rg1 treatment significantly improved cerebral blood flow, locomotion, and limb coordination, reduced ROS production, increased MAP2 and PSD95 expression, and decreased p-Tau, NOX2, p-PLC, CN, NFAT1, and NLRP1 expression. Calcium imaging results showed that Rg1 could inhibit calcium overload and resist the imbalance of calcium homeostasis after OGD/R in HT22 cells.
UNASSIGNED: Rg1 plays a neuroprotective role in attenuating CIRI by inhibiting oxidative stress, calcium overload, and neuroinflammation.
摘要：
■缺血性脑血管病的发病率近年来呈上升趋势，已成为神经功能障碍和死亡的主要原因之一。已发现人参皂苷Rg1在许多神经退行性疾病中具有防止神经元损伤的作用。然而,Rg1对脑缺血再灌注损伤(CIRI)的保护作用和机制尚不完全清楚。这里,我们报道了Rg1治疗对小鼠CIRI的神经保护作用及其可能的机制。
■双侧颈总动脉结扎用于建立小鼠慢性CIRI模型。在OGD/R后用Rg1处理HT22细胞以研究其对[Ca2]i的影响。采用开场试验和爬杆试验检测行为损伤。激光散斑血流流量计用于测量脑血流量。Nissl和H&E染色用于检查神经元损伤。Western印迹用于检查MAP2，PSD95，Tau，p-Tau,NOX2，PLC，p-PLC,CN,NFAT1和NLRP1表达。钙成像用于测试[Ca2]i的水平。
■Rg1治疗可显着改善脑血流量，运动，和肢体协调，减少ROS产生，MAP2和PSD95表达增加，减少p-Tau,NOX2,p-PLC,CN,NFAT1和NLRP1表达。钙成像结果显示,Rg1可抑制HT22细胞OGD/R后钙超载,抵抗钙稳态失衡。
■Rg1通过抑制氧化应激在减轻CIRI中发挥神经保护作用，钙超载，和神经炎症。
■双侧颈总动脉结扎用于建立小鼠慢性CIRI模型。在OGD/R后用Rg1处理HT22细胞以研究其对[Ca2]i的影响。采用开场试验和爬杆试验检测行为损伤。激光散斑血流流量计用于测量脑血流量。Nissl和H&E染色用于检查神经元损伤。Western印迹用于检查MAP2，PSD95，Tau，p-Tau,NOX2，PLC，p-PLC,CN,NFAT1和NLRP1表达。钙成像用于测试[Ca2]i的水平。
■Rg1治疗可显着改善脑血流量，运动，和肢体协调，减少ROS产生，MAP2和PSD95表达增加，减少p-Tau,NOX2,p-PLC,CN,NFAT1和NLRP1表达。钙成像结果显示,Rg1可抑制HT22细胞OGD/R后钙超载,抵抗钙稳态失衡。
■Rg1通过抑制氧化应激在减轻CIRI中发挥神经保护作用，钙超载，和神经炎症。
