Abstract：
In recent years, manganese dioxide (MnO2) nanoparticles with unique physicochemical properties have been widely used in many biomedical fields, such as biosensors, contrast agents, tumor therapy, and drug delivery. From these applications, MnO2 nanoparticles have great clinical translation potential. However, by contrast, the in vitro and in vivo biosafety of MnO2 nanoparticles have been deeply and thoroughly clarified for the clinical translation, which hinders their clinical applications. In this work, we deeply investigated the blood safety of MnO2 nanoparticles by conducting a series of in vitro and in vivo experiments. These included the effects of MnO2 nanoparticles on morphology of red blood cells, activation of platelets, coagulation functions, and toxicity of key organs. The obtained results show that these effects displayed a concentration-dependent manner of MnO2 nanoparticles. Different safe concentration ranges could be found in the different experimental index. This study provides important guidance for the specific biomedical applications of MnO2 nanoparticles, greatly accelerating their laboratory development and clinical translation.
摘要：
近年来,二氧化锰(MnO2)纳米粒子以其独特的物理化学性质被广泛应用于许多生物医学领域,比如生物传感器，造影剂,肿瘤治疗,和药物输送。从这些应用中,MnO2纳米粒子具有伟大的临床翻译潜力。然而,相比之下,MnO2纳米颗粒的体外和体内生物安全性已经深入和彻底地阐明，这阻碍了它们的临床应用。在这项工作中,我们通过进行一系列的体内外实验，深入研究了MnO2纳米颗粒的血液安全性。这些包括MnO2纳米颗粒对红细胞形态的影响，激活血小板，凝血功能，和关键器官的毒性。获得的结果表明，这些效果显示出MnO2纳米颗粒的浓度依赖性。在不同的实验指标中可以找到不同的安全浓度范围。本研究为MnO2纳米颗粒的具体生物医学应用提供了重要指导,大大加快了他们的实验室发展和临床翻译。
