UNASSIGNED: To assess potential role of chrysin and BM-MSC exosomes on ultra-structure, viability and function of human dermal fibroblasts-adult (HDFa) stimulated by LPS.
UNASSIGNED: HDFa cells were divided into: Group I: Cells received no treatment. Group II: Cells were stimulated with LPS. Group III: LPS stimulated cells were treated with chrysin. Group IV: LPS stimulated cells were treated with exosomes.
UNASSIGNED: After 48 h, ultrastructural examination of HDFa cells in Group I revealed intact plasma membrane and numerous cytoplasmic organelles. Group II displayed destructed plasma membrane and apoptotic bodies. Group III showed intact plasma membrane with loss of its integrity at some areas. Group IV demonstrated intact plasma membrane that showed fusion with exosomes at some areas. Statistical analysis of MTT represented highest mean value of cell viability% in Group IV followed by Groups III, I and II respectively. Statistical analysis of enzyme-linked immunosorbent assay (ELISA) showed the highest mean value of interleukin-1β (IL-1β) was in Group II followed by Groups III, IV and I, while highest mean values of interleukin-10 (IL-10), nuclear factor-erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) proteins were in Group I, followed by Groups IV, III and II respectively.
UNASSIGNED: LPS have harmful consequences on ultra-structure, viability and function of HDFa cells. BM-MSC exosomes have better regenerative action on inflamed fibroblasts in comparison to chrysin.
■为了评估chrysin和BM-MSC外泌体对超微结构的潜在作用,LPS刺激的人真皮成纤维细胞(HDFa)的活力和功能。
■HDFa细胞分为:组I:细胞未接受处理。组II:用LPS刺激细胞。组III:用chrysin处理LPS刺激的细胞。IV组:用外泌体处理LPS刺激的细胞。
■48小时后,第一组HDFa细胞的超微结构检查显示完整的质膜和许多细胞质细胞器。第二组显示了破坏的质膜和凋亡体。第III组显示出完整的质膜,在某些区域失去了完整性。第IV组显示完整的质膜,在某些区域显示与外泌体融合。MTT的统计分析代表第IV组的最高细胞活力平均值%,其次是第III组。I和II分别酶联免疫吸附试验(ELISA)统计分析显示,Ⅱ组白细胞介素-1β(IL-1β)平均值最高,其次是Ⅲ组,IV和I,而白细胞介素-10(IL-10)的最高平均值,核因子-红细胞2相关因子2(Nrf2)和血红素加氧酶-1(HO-1)蛋白在I组中,其次是第四组,分别为III和II。
■LPS对超结构有有害影响,HDFa细胞的活力和功能。与chrysin相比,BM-MSC外泌体对发炎的成纤维细胞具有更好的再生作用。