Abstract:
This study investigated the impact of Brochothrix (B.) thermosphacta and Pseudomonas (Ps.) fragi on the transcriptomes of Photobacterium (P.) phosphoreum and P. carnosum on chicken meat under modified atmosphere (MA) and air atmosphere (AA). P. phosphoreum TMW2.2103 responded to MA with a reduced transcript number related to cell division and an enhanced number related to oxidative stress. Concomitantly, the analysis revealed upregulation of fermentation and downregulation of respiration. It predicts enhanced substrate competition in presence of co-contaminants/MA. In contrast, the strain upregulated the respiration in AA, supposably due to improved substrate accessibility in this situation. For P. carnosum TMW2.2149 the respiration was downregulated, and the pyruvate metabolism upregulated under MA. MA/co-contaminant resulted in multiple upregulated metabolic routes. Conversely, AA/co-contaminant resulted only in minor regulations, showing inability to cope with fast growing competitors. Observations reveal different strategies of photobacteria to react to co-contaminants on meat.
摘要:
本研究调查了Brochothrix(B.)热球和假单胞菌(Ps。)光细菌转录组上的片段(P.)在改良气氛(MA)和空气气氛(AA)下对鸡肉的磷酸和肉豆蔻。磷酸假单胞菌TMW2.2103对MA的反应与细胞分裂相关的转录物数量减少,与氧化应激相关的数量增加。同时,分析显示发酵上调和呼吸下调。它预测在共污染物/MA存在下增强的底物竞争。相比之下,该菌株上调了AA的呼吸,可能是由于在这种情况下改善了基材的可及性。对于P.carnosumTMW2.2149,呼吸被下调,丙酮酸代谢在MA下上调。MA/共污染物导致多种上调的代谢途径。相反,AA/共污染物仅导致较小的规定,表现出无法应对快速增长的竞争对手。观察结果揭示了光细菌与肉上的共污染物反应的不同策略。