Abstract：
Spinal cord injury (SCI) will lead to irreversible damage of sensory and motor function of central nervous system, which seriously affects patient\'s quality of life. A variety of nerve engineering materials carrying various stem cells and cell growth factors had used to promote the repair of SCI, but they could not mimic the actual matric niche at spinal cord to promote cell proliferation and differentiation. Thus, developing novel biomaterial providing better niche of spinal cord is a new strategy to treat the severe SCI. In this study, we constructed porcine spinal cord decellularized matrix scaffold (SC-DM) with biocompatibility to load engineered basic fibroblast growth factor-overexpressing human umbilical cord mesenchymal stromal cells (bFGF-HUCMSCs) for treating SCI. The continuously released bioactive bFGF factors from grafted bFGF-HUCMSCs and three-dimensional niche by SC-DM promoted the differentiation of endogenous stem cells into neurons with nerve conduction function, leading a markedly motor function recovery of SCI. These results indicated that the functional bFGF-HUCMSCs/SC-DM scaffold provided more suitable matric niche for nerve cells, that would be a promising strategy for the clinical application of SCI.
摘要：
脊髓损伤会导致中枢神经系统感觉和运动功能的不可逆损伤,严重影响患者的生活质量。多种携带各种干细胞和细胞生长因子的神经工程材料已用于促进SCI的修复，但它们不能模仿脊髓的实际基质生态位促进细胞增殖和分化。因此，开发提供更好的脊髓生态位的新型生物材料是治疗严重SCI的新策略。在这项研究中,我们构建了具有生物相容性的猪脊髓去细胞基质支架（SC-DM），以负载工程化的碱性成纤维细胞生长因子过表达的人脐带间充质基质细胞（bFGF-HUCMSCs）治疗SCI。SC-DM移植的bFGF-HUCMSCs和三维小生境持续释放的生物活性bFGF因子促进内源性干细胞向具有神经传导功能的神经元分化，导致SCI明显的运动功能恢复。这些结果表明，功能性bFGF-HUCMSCs/SC-DM支架为神经细胞提供了更合适的基质生态位，这将是SCI临床应用的一个有希望的策略。
