UNASSIGNED: The present study aimed to evaluate the role of the genetic background of chickens in viral load and the expression level of apoptotic genes in different tissues of two hybrids of commercial broiler chickens (Ross 308 and Cobb 500) challenged with IBV.
UNASSIGNED: Chickens at 21 days of age were nasally challenged with 200 μL of allantoic fluid containing 104 EID50/ml of Iranian variant-2-like IBV (IS/1494). The expression level of apoptotic genes (Fas, FasL, Bax, and Bcl-2) in the tracheal and renal tissues and the amount of viral load in the tracheal, renal, and cloacal swab samples were investigated two, five, and seven days after IBV infection by RT-qPCR assay.
UNASSIGNED: The amount of viral load and apoptotic the expression level of apoptotic genes in the tracheal (two and five days after infection) and renal samples (seven days after infection) were significantly higher in the Ross challenged group than in the Cobb challenged group.Furthermore, no difference was observed in the cloaca viral load on sampling days.
UNASSIGNED: To our knowledge, this is the first report that evaluated the role of the chickens\' genetic background in the amount of viral load and the expression level of apoptotic genes against IBV. Further studies are needed to investigate the pathogenic characteristics of IBV in Ross 308 and Cobb 500 chickens.
■本研究旨在评估鸡的遗传背景在病毒载量中的作用以及凋亡基因在两种商品肉鸡杂种(Ross308和Cobb500)的不同组织中的表达水平受到IBV攻击。
■用200μL尿囊液对21日龄的鸡进行鼻攻击,尿囊液含有104EID50/ml伊朗变体2样IBV(IS/1494)。凋亡基因的表达水平(Fas,FasL,Bax,和Bcl-2)在气管和肾组织中以及气管中的病毒载量,肾,和泄殖腔拭子样本被调查了两个,五,和IBV感染后7天通过RT-qPCR测定。
■气管(感染后2天和5天)和肾脏样本(感染后7天)中的病毒载量和凋亡基因的表达水平明显高于Ross攻击组。此外,采样日的泄殖腔病毒载量无差异.
■据我们所知,这是评估鸡的遗传背景在病毒载量和抗IBV凋亡基因表达水平中的作用的第一份报告。需要进一步研究以调查Ross308和Cobb500鸡的IBV的致病特征。