UNASSIGNED: DNA extraction was performed using five methods (an improved method, Invisorb Spin Plant Mini Kit, EZ-10 Spin Column, Sarrbrucken method (HZI, Germany) and Kirby Bauer\'s method). To evaluate the quantity and quality of extracted genomic DNA, UV absorbance of all samples and efficiency of polymerase chain reaction (PCR) were evaluated.
UNASSIGNED: Overall, the results revealed that the highest quantity (up to 4000 ng/μl) and good quality of DNA was obtained using introduced DNA extraction method.
UNASSIGNED: Results indicated that recently introduced improved method was more efficient for extraction of DNA from actinobacteria for DDH (DNA-DNA hybridization) test and for those require the high concentration of DNA.
■使用五种方法进行DNA提取(一种改进的方法,Invisorb旋转植物迷你套件,EZ-10旋转柱,Sarrbrucken方法(HZI,德国)和柯比·鲍尔的方法)。为了评估提取的基因组DNA的数量和质量,评估所有样品的UV吸光度和聚合酶链反应(PCR)的效率。
■总的来说,结果表明,使用引入的DNA提取方法可以获得最高的DNA数量(高达4000ng/μl)和良好的质量。
■结果表明,最近引入的改进方法对于从放线菌中提取DNA进行DDH(DNA-DNA杂交)测试以及需要高浓度DNA的方法更有效。