METHODS: This is a prospective study, carried out in the Reproductive Medicine and Biology Unit of the Amiens-Picardy University Hospital (France). It was included 14 couples, managed in IVF-ICSI in our center, from January to March 2020. Thirty-eight oocytes, identified as immature after cumulus-oocyte complexes (COC) stripping for ICSI, were placed in our in vitro maturation medium with the addition of autologous cumulus cells. Oocytes that had reached the metaphase II stage after a maximum of 36 hours of maturation were microinjected. The fertilization and embryonic development potential of the in vitro matured oocytes were compared to those of 148 in vivo matured \"siblings\" oocytes from the same oocyte retrieval, and then also compared to those of 127 in vivo matured oocytes from different patients (control group).
METHODS: Maturation rate, fertilization rate, early cleavage rate and developmental activity to blastulation rate.
UNASSIGNED: Embryo quality at cleavage and blastocyst stages, blastulation rate, and useful blastulation rate.
RESULTS: No significant difference was found in the main and secondary criteria of the study compared to the \"siblings\" in vivo matured oocytes from the same oocyte retrieval. However, a significant difference was obtained on the rate of early cleavage and useful blastulation when our cohort was compared to mature in vivo oocytes from different patients (control group).
CONCLUSIONS: This study has shown that after incubation in our in vitro maturation autologous cumulus cell co-culture with cumulus-oocyte cells, immature oocytes recovered during stimulated cycles can give rise to competent oocytes, i.e., capable of being fertilized, of cleaving, and of developing into embryos up to the blastocyst stage. Our study therefore seems to be in the direction of a favorable use of these immature oocytes obtained after stimulated IVF-ICSI cycles. The continuation of this study by including a larger number of oocytes is necessary in order to evaluate the real contribution of this technique in routine.
方法:这是一项前瞻性研究,
方法:成熟率,
未经证实:卵裂和囊胚阶段的胚胎质量,
结果:与来自相同卵母细胞的体内成熟卵母细胞相比,在研究的主要和次要标准中没有发现显着差异。
结论:这项研究表明,在我们体外成熟的自体卵丘细胞与卵丘-卵母细胞共培养后,