Abstract：
The effects of androgens on human skin mainly include the regulation of growth and differentiation of hair follicles and sebaceous glands. Androgens may have some physiological roles in sheep skin that are similar to those of humans, but further confirmation is needed. Therefore, Hetian sheep were chosen in this study as an animal model to explore the effects of testosterone on skin structure and factors related to androgen conversion and binding in Hetian sheep. The sheep were treated with different concentrations of testosterone for 42 days. Skin tissue sections were prepared and then subjected to hematoxylin-eosin, Sacpic, Masson\'s trichrome, and Oil Red O staining to observe changes in skin morphology. Changes in the content of blood-related factors were also detected using ELISA kits. The skin tissue distribution of androgen receptor was explored by immunohistochemistry and immunofluorescence assays. The results showed that testosterone significantly increases the sebaceous gland area and stimulates the formation of new sebaceous glands. Further exploration revealed that testosterone promotes the proliferation of sebaceous gland juvenile cells. However, testosterone was found to have no significant effect on hair follicle density and hair follicle structure. Testosterone increased dihydrotestosterone levels but decreased 5α-reductase 1 and 5α-reductase 2 levels. The androgen receptors were distributed in the hair follicles, sebaceous glands, and some major skin appendages of Hetian sheep. This study suggests that androgens can be effective in regulating sebum production in sheep. This study will help advance research efforts to further explore the molecular and cellular mechanisms by which androgens modify sheep follicles and sebaceous glands.
摘要：
雄激素对人体皮肤的作用主要包括调节毛囊和皮脂腺的生长和分化。雄激素在绵羊皮肤中可能具有一些与人类相似的生理作用，但需要进一步确认。因此,本研究以和田羊为动物模型,探讨睾酮对和田羊皮肤结构及雄激素转化和结合相关因素的影响。用不同浓度的睾酮处理绵羊42天。制备皮肤组织切片，然后接受苏木精-伊红，Sacpic,马森的三色，油红O染色观察皮肤形态变化。还使用ELISA试剂盒检测血液相关因子含量的变化。通过免疫组织化学和免疫荧光测定探索雄激素受体的皮肤组织分布。结果表明，睾酮显著增加皮脂腺面积，刺激新皮脂腺的形成。进一步探索发现，睾酮促进皮脂腺幼年细胞的增殖。然而,发现睾酮对毛囊密度和毛囊结构没有显著影响。睾酮增加了二氢睾酮水平，但降低了5α-还原酶1和5α-还原酶2水平。雄激素受体分布在毛囊中,皮脂腺,和田羊的一些主要皮肤附属物。这项研究表明，雄激素可以有效调节绵羊皮脂的产生。这项研究将有助于推进进一步探索雄激素修饰绵羊卵泡和皮脂腺的分子和细胞机制的研究工作。
