PDF(Pubmed)
Abstract：
In this study, four kinds of chemical substances (2,3,5,6-tetramethylpyrazine, β-ionone, citronellal, and paeonol), three kinds of plant essential oils (tea tree essential oil, lavender essential oil, and myrrh essential oil), and their combinations were selected to explore their synergistic effects on tobacco beetle [Lasioderma serricorne (Fabricius) (Coleoptera: Anobiidae)] adults by the behavioral test and laboratory simulation test. Behavioral test results showed that some of the combinations revealed a synergistic effect on tobacco beetle adults, especially the sexual attractant +2,3,5,6-tetramethylpyrazine + β-ionone + citronellal + paeonol (SABCD, one portion of sexual attractant, and 1 mg/L synergistic substances) combination and the food attractant +2,3,5,6-tetramethylpyrazine + paeonol (FAD, 1 ml of food attractant and 1 mg/L synergistic substances) combination showed the best behavioral effect on tobacco beetle adults with average dwell times of 120.97 and 126.74 s, respectively, compared to those of other combinations. Meanwhile, SABCD had the highest selection rate [89.47%, about 1.5 times that of the sexual attractant (S)] on tobacco beetle adults compared with those of other combinations. In addition, laboratory simulation test results showed that the SABCD combination had the highest average selection rate (37.31%, about 2 times that of S) on tobacco beetle adults at 1 mg/L. However, our results showed that there was no significant difference in the indoor simulation results of food attractant synergistic substances. Our results will provide guidance for the development of new pesticides for tobacco beetle adults.
摘要：
在这项研究中,四种化学物质(2,3,5,6-四甲基吡嗪,β-紫罗兰酮,香茅醛,和paeonol),三种植物精油（茶树精油，薰衣草精油，和没药精油)，通过行为测试和实验室模拟测试，选择它们的组合来探索它们对烟草甲虫[Lasiodermaserricorne（Fabricius）（鞘翅目：Anobiidae）]成虫的协同作用。行为测试结果表明,部分组合对烟草甲虫成虫具有协同作用,尤其是性引诱剂+2,3,5,6-四甲基吡嗪+β-紫罗兰酮+香茅醛+丹皮酚(SAAB,一部分性引诱剂，和1毫克/升的协同物质）组合和食物引诱剂+2,3,5,6-四甲基吡嗪+丹皮酚（FAD，1ml食物引诱剂和1mg/L协同物质）的组合对烟草甲虫成虫的行为效果最佳，平均停留时间为120.97和126.74s，分别,与其他组合相比。同时,选择率最高[89.47%，与其他组合相比，烟草甲虫成虫上的性引诱剂（S）]的约1.5倍。此外,实验室模拟试验结果表明,SAAB组合的平均选择率最高(37.31%,1mg/L时，S）对烟草甲虫成虫的约2倍。然而,我们的结果表明，食品引诱剂协同物质的室内模拟结果没有显着差异。本研究结果将为烟草甲虫成虫新型农药的开发提供指导。
