Abstract：
Nevoid basal cell carcinoma syndrome (NBCCS) associated odontogenic keratocysts (OKCs) show more aggressive behavior and it has a higher frequency of relapse than non-syndromic OKCs. Stromal myofibroblasts (MFs), characterized by α-smooth muscle actin (αSMA), desmin and caldesmon expression, and metalloproteinases (MMPs) have an essential role in the remodeling of the extracellular matrix (ECM). The aim of the study is to analyze the immunohistochemical expression of MMP-7, MMP-9, αSMA and other new markers in the study of OKCs MFs such as desmin and caldesmon in NBCCS-associated OKCs compared to recurrent and sporadic keratocysts. Fourty 40 patients (23 M and 17 F) underwent surgery to remove the OKCs. The histological sections in paraffin were incubated with markers antibodies and a semi-quantitative score was used to evaluate the immunoreactivity. Densitometric analysis showed a very significantly increased expression of αSMA, caldesmon, MMP-7 and MMP-9 in NBCCS-OKCs compared to non-syndromic OKCs (p < 0.001). However, desmin showed a not significant increased expression in non-syndromic OKC compared to NBCCS-OKCs specimens in which desmin was slightly or not at all expressed. NBCSS-OKCs showed a greater distribution of MFs compared to the other OKCs subtypes. Further studies will be needed to evaluate whether the different expressions of these markers can be correlated to a different clinical behavior.
摘要：
痣样基底细胞癌综合征（NBCCS）相关的牙源性角化囊肿（OKC）表现出更积极的行为，并且与非综合征性OKC相比具有更高的复发频率。基质肌成纤维细胞（MFs），以α-平滑肌肌动蛋白(αSMA)为特征，desmin和caldesmon表达式,金属蛋白酶（MMPs）在细胞外基质（ECM）的重塑中具有重要作用。该研究的目的是分析与复发性和散发性角化囊肿相比，在NBCCS相关OKCs中的desmin和caldesmon等OKCsMF研究中MMP-7，MMP-9，αSMA和其他新标志物的免疫组织化学表达。40名患者（23M和17F）接受了手术切除OKC。将石蜡中的组织切片与标记抗体一起孵育,并使用半定量评分来评估免疫反应性。光密度分析显示αSMA表达显著增加,Caldesmon,NBCCS-OKC中的MMP-7和MMP-9与非综合征型OKC相比(p<0.001)。然而,与NBCCS-OKC标本相比，结蛋白在非综合征性OKC中的表达没有显着增加，其中结蛋白略有表达或根本不表达。与其他OKC亚型相比，NBCSS-OKC显示出更大的MF分布。需要进一步的研究来评估这些标志物的不同表达是否可以与不同的临床行为相关。
