Abstract：
The guava fruit fly, Bactrocera correcta, is one of the most destructive pests in the genus Bactrocera and detects environmental odorants mainly through antennal olfactory sensilla phenotypes with nanopores. However, it is unclear whether there are naturally occurring abnormal antennal olfactory sensilla phenotypes that affect olfaction. Here, we found that there were abnormal bulges besides nanopores on the surface of trichoid and basiconic olfactory sensilla in the antennal flagellum of long-term laboratory rearing colony (LTC), and that nanopore number in these olfactory sensilla was also remarkably reduced. Notably, the electroantennogram (EAG) responses of LTC insects to methyl eugenol or β-caryophyllene were inhibited, and their behavioral responses elicited by the same odorants were also impaired. These results revealed naturally occurring abnormal antennal olfactory sensilla phenotypes which were involved in olfactory deficit in B. correcta, providing a platform to further study nanopore-targeted pest control technologies in the future.
摘要：
番石榴果蝇,正杆菌,是Bactrocera属中最具破坏性的害虫之一，主要通过具有纳米孔的触角嗅觉表型来检测环境气味。然而,目前尚不清楚是否有自然发生的异常触角嗅觉表型影响嗅觉。这里,我们发现，在长期实验室饲养菌落（LTC）的触角鞭毛中，除了毛状和基底嗅觉感官表面的纳米孔外，还有异常的凸起，这些嗅觉传感器中的纳米孔数量也显著减少。值得注意的是,LTC昆虫对甲基丁香酚或β-石竹烯的触角图（EAG）反应受到抑制，相同气味引起的行为反应也受到损害。这些结果揭示了自然发生的异常触角嗅觉表型，这些表型与正双歧杆菌的嗅觉缺陷有关，为今后进一步研究纳米孔靶向害虫防治技术提供平台。
