Abstract：
The antimicrobial activity of ε-poly-l-lysine (EPL) has been documented, but its antifungal activity on yeast is not well defined and its mechanism of action has been vaguely explained. Our studies revealed that on both, Candida albicans and Saccharomyces cerevisiae, the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were 250 μg·mL-1; EPL produced a K+ and Ca2+ efflux, and at higher concentrations also an efflux of material absorbing at 260 nm, small peptides, and phosphate is produced, along with the inhibition of fermentation and extracellular acidification and respiration. Moreover, growth was inhibited, reactive oxygen species (ROS) production increased, and cell viability decreased. The polycation also produced plasma membrane potential hyperpolarization. The effects were dependent both on the cell quantity and polycation concentration, as well as the media used. The plasma membrane disruption was confirmed by TEM and PI staining.
摘要：
ε-聚-1-赖氨酸（EPL）的抗菌活性已被证明，但其对酵母的抗真菌活性尚不明确，其作用机制也有模糊的解释。我们的研究表明，在这两种情况下，白色念珠菌和酿酒酵母，最低抑菌浓度(MIC)和最低杀菌浓度(MFC)为250μg·mL-1；EPL产生K+和Ca2+外排，在更高的浓度下，在260nm处吸收的材料也会流出，小肽，产生磷酸盐，随着发酵和细胞外酸化和呼吸的抑制。此外，生长受到抑制，活性氧（ROS）产量增加，细胞活力下降。聚阳离子还产生了质膜电位超极化。影响取决于细胞数量和聚阳离子浓度，以及使用的媒体。通过TEM和PI染色确认质膜破裂。
