PDF(Pubmed)
Abstract：
UNASSIGNED: Caveolae are plasma membrane subdomains of many mammalian cells that play critical roles in cellular processes, including endocytosis, signal transduction and tumorigenesis. Cavin proteins are essential for caveola formation, structure and function and are reported to be involved in various human diseases, but little is known about their expression and prognostic value in leukemia.
UNASSIGNED: We performed a detailed analysis of Cavin family mRNA expression levels in different cancer tissues vs. normal tissues via the ONCOMINE, Gene Expression Profiling Interactive Analysis (GEPIA) and Cancer Cell Line Encyclopedia (CCLE) databases. Then, we used qRT-PCR and Western blotting to validate Cavin1-4 expression in 10 fresh leukemia samples. Moreover, we estimated their prognostic value in leukemia with the R programming language and GEPIA database.
UNASSIGNED: The expression of Cavin members is low in most human cancers, especially in leukemia. Cavin-1 and Cavin-2 are often more expressed in myeloid leukemia than lymphoblastic leukemia, but Cavin-4 has the opposite pattern. Interestingly, low expression of CAVIN1 and CAVIN4 is correlated with poorer outcome but low CAVIN2 expression is associated with a significantly better leukemia prognosis in leukemia.
UNASSIGNED: The Cavin family showed significant expression differences between leukemia and normal cells. High Cavin-2 and low Cavin-4 levels predict poor survival and could be promising subtype diagnosis and prognosis biomarkers for leukemia.
UNASSIGNED: We performed a detailed analysis of Cavin family mRNA expression levels in different cancer tissues vs. normal tissues via the ONCOMINE, Gene Expression Profiling Interactive Analysis (GEPIA) and Cancer Cell Line Encyclopedia (CCLE) databases. Then, we used qRT-PCR and Western blotting to validate Cavin1-4 expression in 10 fresh leukemia samples. Moreover, we estimated their prognostic value in leukemia with the R programming language and GEPIA database.
UNASSIGNED: The expression of Cavin members is low in most human cancers, especially in leukemia. Cavin-1 and Cavin-2 are often more expressed in myeloid leukemia than lymphoblastic leukemia, but Cavin-4 has the opposite pattern. Interestingly, low expression of CAVIN1 and CAVIN4 is correlated with poorer outcome but low CAVIN2 expression is associated with a significantly better leukemia prognosis in leukemia.
UNASSIGNED: The Cavin family showed significant expression differences between leukemia and normal cells. High Cavin-2 and low Cavin-4 levels predict poor survival and could be promising subtype diagnosis and prognosis biomarkers for leukemia.
摘要：
■Caveolae是许多哺乳动物细胞的质膜亚结构域，在细胞过程中起关键作用，包括内吞作用,信号转导和肿瘤发生。Cavin蛋白是形成Caveola所必需的,结构和功能，据报道参与各种人类疾病，但对它们在白血病中的表达和预后价值知之甚少。
■我们详细分析了不同癌组织中Cavin家族mRNA表达水平与通过OCOMINE的正常组织，基因表达谱交互式分析(GEPIA)和癌细胞系百科全书(CCLE)数据库。然后，我们使用qRT-PCR和Western印迹验证了10例新鲜白血病样本中Cavin1-4的表达。此外，我们使用R编程语言和GEPIA数据库评估了它们在白血病中的预后价值.
■在大多数人类癌症中，Cavin成员的表达很低，尤其是白血病。Cavin-1和Cavin-2在髓细胞性白血病中的表达通常高于淋巴母细胞性白血病。但Cavin-4有相反的模式.有趣的是,CAVIN1和CAVIN4低表达与预后较差相关,但CAVIN2低表达与白血病预后显著改善相关.
■Cavin家族在白血病细胞和正常细胞之间显示出显着的表达差异。高Cavin-2和低Cavin-4水平预测生存不良，可能是有希望的白血病亚型诊断和预后生物标志物。
■我们详细分析了不同癌组织中Cavin家族mRNA表达水平与通过OCOMINE的正常组织，基因表达谱交互式分析(GEPIA)和癌细胞系百科全书(CCLE)数据库。然后，我们使用qRT-PCR和Western印迹验证了10例新鲜白血病样本中Cavin1-4的表达。此外，我们使用R编程语言和GEPIA数据库评估了它们在白血病中的预后价值.
■在大多数人类癌症中，Cavin成员的表达很低，尤其是白血病。Cavin-1和Cavin-2在髓细胞性白血病中的表达通常高于淋巴母细胞性白血病。但Cavin-4有相反的模式.有趣的是,CAVIN1和CAVIN4低表达与预后较差相关,但CAVIN2低表达与白血病预后显著改善相关.
■Cavin家族在白血病细胞和正常细胞之间显示出显着的表达差异。高Cavin-2和低Cavin-4水平预测生存不良，可能是有希望的白血病亚型诊断和预后生物标志物。
