PDF(Pubmed)
Abstract：
Understanding whether and how melatonin (MT) may impact sheep oocyte development competence is central to our ability to predict how sheep oocytes will respond to artificially regulated estrus. Implanting MT can make sheep enter estrus during the non-breeding season. One study found that the blastocyst rate increased under MT treatment, while another found that the blastocyst rate decreased. Therefore, we conducted a meta-analysis of MT directly and indirectly influencing sheep oocytes. A total of 433 articles were collected from which 20 articles and 34 treatments were finally selected. A method for estimating the default value was established for the litter size analysis. We found that exogenous MT add into in vitro maturation medium was positively related to the blastocyst rate in the lab. However, subcutaneous implanting MT did not affect the in vivo ovulation rate, fertilization rate, blastocyst rate, or pregnancy rate at farm. MT did not affect the in vitro cleavage rate. However, MT improved the in vivo cleavage rate. We hypothesized that implanted MT could increase the concentration of MT in oviduct fluid in vivo, and also that in vitro MT could increase the early cleavage rate of sheep zygotes without affecting the total cleavage rate. In the analysis of oocyte apoptosis caused by injury, the results suggested that pyroptosis would be more suitable for further research. MT produces responses in all body organs, and thus implanting of MT during non-breeding seasons should consider the effect on animal welfare.
摘要：
了解褪黑激素（MT）是否以及如何影响绵羊卵母细胞的发育能力，对于我们预测绵羊卵母细胞如何对人为调节的发情做出反应的能力至关重要。植入MT可以使绵羊在非繁殖季节进入发情期。一项研究发现，在MT治疗下，囊胚率增加，而另一个发现囊胚率下降。因此,我们对MT直接和间接影响绵羊卵母细胞进行了荟萃分析.共收集到433篇文章，最终从中选择了20篇文章和34种处理方法。建立了一种估算垫料大小分析默认值的方法。我们发现在体外成熟培养基中添加外源MT与囊胚率呈正相关。然而,皮下植入MT不影响体内排卵率，受精率,囊胚率,或农场的怀孕率。MT不影响体外裂解率。然而,MT提高了体内卵裂率。我们假设植入的MT可以增加体内输卵管液中MT的浓度，体外MT可以提高绵羊受精卵的早期卵裂率，而不影响总卵裂率。在分析由损伤引起的卵母细胞凋亡，结果提示焦化凋亡更适合进一步研究。MT在所有身体器官中产生反应，因此，在非繁殖季节植入MT应考虑对动物福利的影响。
