PDF(Pubmed)
Abstract：
Pathogenesis-related (PR) proteins play important roles in plant defense response and systemic acquired resistance (SAR). PR1 has antifungal activity against many plant pathogens. In our previous study, RNA sequencing (RNA-seq) was conducted on resistant wheat line TcLr19 and sensitive wheat cultivar Chinese Spring inoculated with Puccinia triticina (Pt) race PHNT. In this study, seven salicylic acid (SA)-induced TaPR1 genes involved in plant disease resistance were found in the RNA-seq library. Quantitative PCR (qPCR) results showed that TaPR1-4 was most induced by Pt among these seven TaPR1 genes in the incompatible interaction. Yeast two-hybrid (Y2H) results showed that TaPR1-4 interacted with TaTLP1 via the αIV helix. Protein-mediated phenotyping assays in vivo and antifungal activity in vitro demonstrated that wheat leaves infiltrated with pure TaPR1-4 protein developed significantly less disease compared to control leaves. This effect was correlated with a strong increase in defense gene expression, and resistance activity was dependent on the CAPE1 motif located in the C-terminal region of TaPR1-4. These findings increase current knowledge regarding the interaction of TaPR1 and TaTLP1 and provide new insights on the role of TaPR1 protein in the resistance of wheat to Pt.
摘要：
发病相关蛋白（PR）在植物防御反应和系统获得性抗性（SAR）中起重要作用。PR1对许多植物病原体具有抗真菌活性。在我们之前的研究中,对抗小麦品系TcLr19和敏感小麦品种中国春季接种了小麦锈菌（Pt）品种PHNT进行了RNA测序（RNA-seq）。在这项研究中,在RNA-seq文库中发现了七个水杨酸（SA）诱导的与植物抗病性有关的TaPR1基因。定量PCR（qPCR）结果表明，在这七个TaPR1基因中，TaPR1-4在不相容相互作用中被Pt诱导的最多。酵母双杂交（Y2H）结果表明，TaPR1-4通过αIV螺旋与TaTLP1相互作用。体内蛋白质介导的表型测定和体外抗真菌活性表明，与对照叶片相比，用纯TaPR1-4蛋白浸润的小麦叶片发生的病害明显减少。这种效应与防御基因表达的强烈增加有关，并且抗性活性取决于位于TaPR1-4C末端区域的CAPE1基序。这些发现增加了有关TaPR1和TaTLP1相互作用的最新知识，并为TaPR1蛋白在小麦对Pt的抗性中的作用提供了新的见解。
