Abstract：
The gut microbiota consists a diverse and complex ecosystem that is involved in beneficial functions as well as potentially harmful conditions for human. Blastocystis sp. is a common parasite of the digestive tract of animals and humans; however, limited data is available concerning the association of asymptomatic Blastocystis infection and gut bacteria composition. Hence, in this cross-sectional study, the gut bacteria composition of twenty asymptomatic Blastocystis sp. positive and twenty Blastocystis sp. negative individuals was assessed with real time PCR. The case and control groups were matched for age and sex. Both groups were negative for other gastrointestinal infections and did not have any gastrointestinal symptoms. The subtype of ten Blastocystis sp. isolates was assessed based on sequencing. Sequencing of ten Blastocystis sp. isolates revealed the ST1, ST2, and ST3 subtypes in 40%, 30%, and 30% of the isolates. The relative expression of each bacteria in the case than control group revealed that the expression level of Bifidobacterium group (P < 0.033), Peptostreptococcus productus (P < 0.014), Lactobacillus/Enterococcus group (P < 0.001), and Escherichia coli (P < 0.001) were significantly upregulate in the Blastocystis sp. carriers than the control group, while the relative amounts of Bacteroides fragilis (P < 0.001) and Enterococcus sp. (P < 0.001) were significantly downregulated in the case than the control group. Taken together, the results of this study have shown that asymptomatic Blastocystis infection could alter the composition of gut bacteria in healthy individuals.
摘要：
肠道微生物群由一个多样化和复杂的生态系统组成，涉及人类的有益功能以及潜在的有害条件。囊胚。是动物和人类消化道的常见寄生虫；然而，关于无症状芽囊原虫感染和肠道细菌组成的关联的数据有限.因此，在这项横断面研究中，20个无症状囊胚的肠道细菌组成。阳性和二十个囊胚。阴性个体通过实时PCR进行评估。病例组和对照组的年龄和性别相匹配。两组均为其他胃肠道感染阴性，无任何胃肠道症状。十种囊胚的亚型。基于测序评估分离株。十个囊胚的测序。分离株显示了40%的ST1、ST2和ST3亚型，30%,和30%的分离株。各细菌的相对表达量均高于对照组,显示双歧杆菌组的表达水平(P<0.033),产生的肽链球菌(P<0.014),乳酸杆菌/肠球菌群(P<0.001),和大肠杆菌（P<0.001）在囊胚菌中显着上调。携带者比对照组，而脆弱拟杆菌(P<0.001)和肠球菌的相对量。(P<0.001)均明显下调的病例比对照组。一起来看,这项研究的结果表明，无症状囊胚感染可以改变健康个体的肠道细菌组成。
