Abstract：
A Gram-strain-negative, rod-shaped, aerobic bacterium, designated strain TRM 85114T, was isolated from the Jincaotan wetland in the Pamir Plateau of China. This strain grew optimally at 30 °C and pH 6.0 in the presence of 3% (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain TRM 85114T was affiliated with the genus Halomonas, and shared high sequence similarity with Halomonas korlensis XK1T (97.3%) and Halomonas tibetensis pyc13T (96.4%). Strain TRM 85114T contained C16:0 and C19:0 cyclo ω8c as primary cellular fatty acids, Q-9 as predominate respiratory quinone, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phospholipids of unknown structure containing glucosamine, unidentified aminophospholipids, unidentified lipids and three unidentified phospholipids as the major polar lipids. The complete genome of TRM 85114T comprised 3,902 putative genes with a total of 4,126,476 bp and a G + C content of 61.6%. The average nucleotide identity and digital DNA-DNA hybridization values between strain TRM 85114T and related type Halomonas strains of H. korlensis XK1T, H. tibetensis pyc13T, Chromohalobacter salexigens DSM 6768T, and Halomonas urumqiensis BZ-SZ-XJ27T were 75.4-88.9% and 22.9-39.2%, respectively. Based on phenotypic, chemotaxonomic, and molecular features, strain TRM 85114T represents a novel species of the genus Halomonas, for which the name is proposed as Halomonas jincaotanensis sp. nov.. The type strain is TRM 85114T (CCTCC AB 2021006T = LMG 32311T). The amount of 1-naphthylamine degradation by strain TRM 85114T reached up to 32.0 mg/L in 14 days.
摘要：
革兰氏应变阴性，杆状,需氧细菌，指定菌株TRM85114T，是从中国帕米尔高原的金草滩湿地中分离出来的。该菌株在3%(w/v)NaCl存在下在30°C和pH6.0下最佳生长。16SrRNA基因序列的系统发育分析表明，菌株TRM85114T与Halomonas属相关，并与KorlensisHalomonasXK1T（97.3％）和西藏Halomonaspyc13T（96.4％）具有很高的序列相似性。菌株TRM85114T含有C16：0和C19：0环状ω8c作为主要的细胞脂肪酸，Q-9作为主要的呼吸醌，二磷脂酰甘油，磷脂酰甘油，磷脂酰乙醇胺，未知结构的磷脂含有氨基葡萄糖，不明的氨基磷脂，未知的脂质和三种未知的磷脂是主要的极性脂质。TRM85114T的完整基因组包含3,902个推定基因，总计4,126,476bp，GC含量为61.6％。TRM85114T菌株与相关类型的H.korlensisXK1T之间的平均核苷酸同一性和数字DNA-DNA杂交值，H.西藏pyc13T，嗜血卤菌DSM6768T,和白单胞菌BZ-SZ-XJ27T分别为75.4-88.9％和22.9-39.2％，分别。基于表型，化学分类学，和分子特征，菌株TRM85114T代表了盐单胞菌属的一种新物种，因此，该名称被提议为金草酸盐。11月。.菌株类型为TRM85114T(CCTCCAB2021006T=LMG32311T)。菌株TRM85114T降解1-萘胺的量在14天内达到32.0mg/L。
