UNASSIGNED: A total of 120 fresh fecal samples, including 19 species of NHPs, were collected from four breeding bases of the zoo from May to June 2019. The infections of six species of Entamoeba spp. were detected by PCR using the 16S or 18S rDNA-specific primers, and the positive samples were sequenced and analyzed.
UNASSIGNED: Entamoeba spp. were detected as positive in 59 NHPs fecal samples (49.17%), including five Entamoeba species: Entamoeba histolytica (7.50%), E. dispar (22.50%), E. coli (22.50%), E. chattoni (10.00%) and E. nuttalli (1.67%). Infection with one Entamoeba species was more common (35%) than co-infections (13.33%) or infections with three Entamoeba species (0.83%). There was a significantly higher prevalence rate of Entamoeba spp. in the species Pongo pygmaeus and Macaca mulatta than in Papio sp., Mandrillus sphinx, and Saimiri sciureus.
UNASSIGNED: Entamoeba spp. are highly prevalent in the NHPs raised in Nanjing Hongshan Forest Zoo. Therefore, attention should be paid to the development of containment strategies of Entamoeba spp. in this zoological garden.
■总共120个新鲜粪便样本,包括19种NHPs,于2019年5月至6月从动物园的四个繁殖基地收集。6种内阿米巴属的感染。使用16S或18SrDNA特异性引物通过PCR检测,并对阳性样本进行测序和分析。
■内变形虫属。在59个NHP粪便样本中检测为阳性(49.17%),包括五个内阿米巴物种:溶组织内阿米巴(7.50%),E.dispar(22.50%),大肠杆菌(22.50%),E、查托尼(10.00%)和纳塔利(1.67%)。一种内阿米巴物种的感染比共感染(13.33%)或三种内阿米巴物种的感染(0.83%)更常见(35%)。内阿米巴的患病率明显较高。在Pongopygmaeus和猕猴中,比Papiosp.,曼陀罗,和Saimirisciureus.
■内变形虫属。在南京红山森林动物园饲养的NHP中非常普遍。因此,应注意制定Entamoebaspp的遏制策略。在这个动物园里。