Abstract：
BACKGROUND: Preeclampsia (PE) is a serious complication of pregnancy, with a global incidence of about 2%-8%. It is one of the important causes of morbidity and mortality among the pregnant women and perinatal infants. Circular RNA (circRNA) has been confirmed to play an important regulatory role in PE. This study aimed to evaluate the role of hsa_circ_0008726 in the occurrence and development of PE.
METHODS: The expression of hsa_circ_0008726 in PE placental tissue and blood was detected by qRT-PCR. CCK-8, wound closure, and Transwell assay were used to measure cell proliferation, migration, and invasion. Bioinformatics prediction, Western blotting, and dual-luciferase reporter gene detection were used to explore the mechanism of hsa_circ_0008726 in HTR8/SVneo cells.
RESULTS: The expression level of circ_0008726 in the placental tissue and blood samples of PE patients was significantly higher than that of normal controls. The overexpression of circ_0008726 can significantly inhibit the proliferation, migration, and invasion ability of HTR-8/SVneo cells. While the silence of circ_0008726 showed an opposite effect. Furthermore, hsa_circ_0008726 can modulate the expression of LHX6 by adsorbing miR-1290.
CONCLUSIONS: Hsa_circ_000872 can regulate LHX6 by adsorbing miR-1290 to inhibit PE progression, thus establishing hsa_circ_000872 as a potential target for predicting and treating PE.
METHODS: The expression of hsa_circ_0008726 in PE placental tissue and blood was detected by qRT-PCR. CCK-8, wound closure, and Transwell assay were used to measure cell proliferation, migration, and invasion. Bioinformatics prediction, Western blotting, and dual-luciferase reporter gene detection were used to explore the mechanism of hsa_circ_0008726 in HTR8/SVneo cells.
RESULTS: The expression level of circ_0008726 in the placental tissue and blood samples of PE patients was significantly higher than that of normal controls. The overexpression of circ_0008726 can significantly inhibit the proliferation, migration, and invasion ability of HTR-8/SVneo cells. While the silence of circ_0008726 showed an opposite effect. Furthermore, hsa_circ_0008726 can modulate the expression of LHX6 by adsorbing miR-1290.
CONCLUSIONS: Hsa_circ_000872 can regulate LHX6 by adsorbing miR-1290 to inhibit PE progression, thus establishing hsa_circ_000872 as a potential target for predicting and treating PE.
摘要：
背景：先兆子痫（PE）是妊娠的严重并发症，全球发病率约为2%-8%。它是孕妇和围生儿发病和死亡的重要原因之一。环状RNA(circularRNA,circRNA)已被证实在PE中起重要的调节作用。本研究旨在评估hsa_circ_0008726在PE发生发展中的作用。
方法：采用qRT-PCR检测PE胎盘组织和血液中hsa_circ_0008726的表达。CCK-8,伤口闭合,和Transwell分析用于测量细胞增殖，迁移,和入侵。生物信息学预测，西方印迹,并通过双荧光素酶报告基因检测来探讨hsa_circ_0008726在HTR8/SVneo细胞中的作用机制。
结果：PE患者胎盘组织和血液样本中circ_0008726的表达水平明显高于正常对照组。circ_0008726的过表达能显著抑制其增殖,迁移,HTR-8/SVneo细胞的侵袭能力。而circ_0008726的沉默显示出相反的效果。此外，hsa_circ_0008726可以通过吸附miR-1290调节LHX6的表达。
结论：Hsa_circ_000872可以通过吸附miR-1290来调节LHX6以抑制PE进展，从而将hsa_circ_000872确立为预测和治疗PE的潜在目标。
方法：采用qRT-PCR检测PE胎盘组织和血液中hsa_circ_0008726的表达。CCK-8,伤口闭合,和Transwell分析用于测量细胞增殖，迁移,和入侵。生物信息学预测，西方印迹,并通过双荧光素酶报告基因检测来探讨hsa_circ_0008726在HTR8/SVneo细胞中的作用机制。
结果：PE患者胎盘组织和血液样本中circ_0008726的表达水平明显高于正常对照组。circ_0008726的过表达能显著抑制其增殖,迁移,HTR-8/SVneo细胞的侵袭能力。而circ_0008726的沉默显示出相反的效果。此外，hsa_circ_0008726可以通过吸附miR-1290调节LHX6的表达。
结论：Hsa_circ_000872可以通过吸附miR-1290来调节LHX6以抑制PE进展，从而将hsa_circ_000872确立为预测和治疗PE的潜在目标。
