METHODS: The expression of hsa_circ_0008726 in PE placental tissue and blood was detected by qRT-PCR. CCK-8, wound closure, and Transwell assay were used to measure cell proliferation, migration, and invasion. Bioinformatics prediction, Western blotting, and dual-luciferase reporter gene detection were used to explore the mechanism of hsa_circ_0008726 in HTR8/SVneo cells.
RESULTS: The expression level of circ_0008726 in the placental tissue and blood samples of PE patients was significantly higher than that of normal controls. The overexpression of circ_0008726 can significantly inhibit the proliferation, migration, and invasion ability of HTR-8/SVneo cells. While the silence of circ_0008726 showed an opposite effect. Furthermore, hsa_circ_0008726 can modulate the expression of LHX6 by adsorbing miR-1290.
CONCLUSIONS: Hsa_circ_000872 can regulate LHX6 by adsorbing miR-1290 to inhibit PE progression, thus establishing hsa_circ_000872 as a potential target for predicting and treating PE.
方法:采用qRT-PCR检测PE胎盘组织和血液中hsa_circ_0008726的表达。CCK-8,伤口闭合,和Transwell分析用于测量细胞增殖,迁移,和入侵。生物信息学预测,西方印迹,并通过双荧光素酶报告基因检测来探讨hsa_circ_0008726在HTR8/SVneo细胞中的作用机制。
结果:PE患者胎盘组织和血液样本中circ_0008726的表达水平明显高于正常对照组。circ_0008726的过表达能显著抑制其增殖,迁移,HTR-8/SVneo细胞的侵袭能力。而circ_0008726的沉默显示出相反的效果。此外,hsa_circ_0008726可以通过吸附miR-1290调节LHX6的表达。
结论:Hsa_circ_000872可以通过吸附miR-1290来调节LHX6以抑制PE进展,从而将hsa_circ_000872确立为预测和治疗PE的潜在目标。