Abstract：
Chrysosplenium is the main component of a variety of Tibetan prescription preparations. Nevertheless, there are few chemical reports for different species of Chrysosplenium, which should be further explored. To this end, ultra-performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry (UPLC-Q Exactive Orbitrap HRMS) and high-performance liquid chromatography-diode array detection (HPLC-DAD) were first integrated to qualitatively analyse the chemical characteristics of Chrysosplenium nudicaule, Chrysosplenium carnosum, Chrysosplenium sikangense, Chrysosplenium griffithii, Chrysosplenium absconditicapsulum, Chrysosplenium forrestii and Chrysosplenium axillare. As a result, a total of 40 compounds were identified or tentatively identified from these 7 species of Chrysosplenium, including 21 flavonoids, 3 triterpenoids and a variety of alkaloids, organic acids and anthraquinones, etc. Among them, 6 compounds were detected for the first time, and 8 compounds are common components in all 7 species of Chrysosplenium. In the specific chromatogram, 4 characteristic peaks, namely Riboflavin, 5,4\'-dihydroxy-3,6,3\'-trimethoxyflavin-7-O-β-D-glucoside, 5,7,3\'-trihydroxy-6,4\',5\'-trimethoxyflavone and Chrysosplenetin, were selected to evaluate the similarities of 17 batches of Chrysosplenium samples, which ranged from 0.770 to 0.994. The established method is simple, feasible and accurate, and was proven to be suitable for characterizing the chemical compositions of Chrysosplenium from different species and evaluating their similarities by specific chromatogram analysis to clarify the rationality of using Chrysosplenium from different species in clinical medication, which provides experimental data for further quality evaluation of Chrysosplenium.
摘要：
金硒是多种藏方制剂的主要成分。然而，很少有不同种类的Chrysosoplenium的化学报告，这应该进一步探讨。为此,首先将超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(UPLC-QExactiveOrbitrapHRMS)和高效液相色谱-二极管阵列检测(HPLC-DAD)相结合，对金黄的化学特性进行定性分析，肉质金，Chrysosopleniumsikangense,Chrysopleniumgriffithii,Chrysosspleniumabscondicapsulum,劳氏金氏和axillare金氏金氏。因此,从这7种金黄属植物中鉴定或初步鉴定出40种化合物，包括21种类黄酮，3三萜类和多种生物碱，有机酸和蒽醌,等。其中,首次检测到6个化合物,和8种化合物是所有7种Chrysoplenium中的常见成分。在特定的色谱图中，4个特征峰,即核黄素,5,4'-二羟基-3,6,3'-三甲氧基黄素-7-O-β-D-葡萄糖苷，5,7,3\'-三羟基-6,4\',5'-三甲氧基黄酮和Chrysopcletin，被选中评估17批金黄样品的相似性，范围从0.770到0.994。所建立的方法简单,可行和准确，并被证明适用于表征来自不同物种的Chrysoplenium的化学成分，并通过特定的色谱图分析评估它们的相似性，以阐明在临床药物中使用来自不同物种的Chrysoplenium的合理性，为进一步评价金黄的质量提供了实验数据。
