PDF(Pubmed)
Abstract：
In the present study, we reported the complete mitogenome sequence of Caenorhabditis tribulationis Stevens & Félix 2019. The whole mitogenome of C. tribulationis is 14006 bp in length with an extreme bias of high AT content (75.26%) (GenBank accession no. OL362111). The mitochondrial genome contains 12 protein-coding genes (PCGs), 22 transfer RNA (tRNAs) genes, 2 ribosomal RNA (12S rRNA and 16S rRNA) genes, and a control region. All genes were unidirectionally transcribed on the same strand, typical for other nematode mitogenomes. 9 PCGs were initiated by typical ATN codons, except for NAD2, CYTB and NAD4, which were start with TTG codons. All the PCGs were predicted to use the typical TAN as the stop codons. The phylogenetic analysis showed that the relationship of C. tribulationis is very close to other species in the family Rhabditidae and separated form species of the families Ascarididae, Toxocaridae, Anisakidae and Ascaridiidae with high bootstrap value support.
摘要：
在本研究中,我们在Stevens&Félix2019年报告了三角隐杆线虫的完整有丝分裂基因组序列.C.trubulationis的整个有丝分裂基因组的长度为14006bp，具有高AT含量（75.26％）的极端偏向（GenBank登录号。OL362111)。线粒体基因组包含12个蛋白质编码基因（PCGs），22个转移RNA（tRNA）基因，2核糖体RNA（12SrRNA和16SrRNA）基因，和控制区。所有基因都在同一条链上单向转录，其他线虫有丝分裂基因组的典型特征。9个PCG由典型的ATN密码子启动，除NAD2、CYTB和NAD4以TTG密码子开头外。预测所有PCG使用典型的TAN作为终止密码子。系统发育分析表明，毛虫的关系与横纹肌科中的其他物种非常接近，并且与A科的分离形式物种非常接近。弓形虫科,具有高bootstrap值的钩虫科和a虫科。
