UNASSIGNED: The in vitro assay for glucose uptake was performed in the baker yeast whereas in-silico study involved retrieving the phytoconstituents from open sources, and predicting for probable targets of diabetes followed by drug-likeness score, probable side effects, and ADMET profile. Homology modeling was performed to construct the target protein glucose transporter-2. In addition, the binding affinity of each ligand with glucose transporter was predicted using AutoDock 4.2.
UNASSIGNED: A total of 17 phytoconstituents from F. benghalensis were identified to possess the anti-diabetic effects. Among them, 4-methoxybenzoic acid scored the highest drug-likeness score and lupeol acetate had the maximum binding affinity of -8.02 kcal/mol with 9 pi-interactions via Tyr324, Phe323, Ile319, Ile200, Ile28, Phe24, and Ala451. Similarly, the extract showed the highest glucose uptake efficacy in yeast cells at 500 µg/mL.
UNASSIGNED: Herein the present study reflected the probable activity of the phytoconstituents from F. benghalensis in promoting the glucose uptake via the in silico and in vitro approaches.
UNASSIGNED:在面包酵母中进行了葡萄糖摄取的体外测定,而计算机研究涉及从开放来源中检索植物成分,
UNASSIGNED:鉴定出来自F.benghalensis的总共17种植物成分具有抗糖尿病作用。
UNASSIGNED:在此,本研究反映了F.benghalensis的植物成分通过计算机和体外方法促进葡萄糖吸收的可能活性。