Abstract：
Sorbitol is known as a biomarker for the evaluation of the progress of diabetic complications. We have developed a sorbitol biosensor using an optical fiber for rapid diagnosis and pathological evaluation of diabetic complications. In this paper, we measured blood sorbitol in diabetic rats using an improved biosensor, and discussed the effectiveness of the developed biosensor and the significance of sorbitol measurement. In order to investigate the effectiveness of the developed biosensor, the blood sorbitol level of type II diabetic rats prepared by streptozotocin administration was measured with the developed sensor. The values of sorbitol were highly correlated with the values measured by the F-kit of food analysis and that we confirmed the sorbitol concentration could be quantified using the developed biosensor. Furthermore, the aldose reductase inhibitor \"eparlrestat\", which is a therapeutic drug that suppresses the accumulation of sorbitol, was administered to diabetic rats, and the blood sorbitol level was measured with the developed biosensor. As a result, the blood glucose level was high in both the treated group and the non-treated group, but the blood sorbitol level in the treated group decreased. The results suggest that the measurement of the sorbitol level with the developed biosensor in addition to the blood glucose level enables evaluation of complications like diabetic neuropathy. In the future, we expected that the developed sorbitol biosensor will be miniaturized, the pretreatment method for blood samples will be simplified, and it will be applied to the development of therapeutic agents for diabetic complications and personalized medicine.
摘要：
山梨醇被认为是评估糖尿病并发症进展的生物标志物。我们已经开发了一种使用光纤的山梨糖醇生物传感器，用于糖尿病并发症的快速诊断和病理评估。在本文中,我们使用改进的生物传感器测量糖尿病大鼠的血液山梨糖醇，并讨论了所开发的生物传感器的有效性和山梨醇测量的意义。为了研究开发的生物传感器的有效性，用开发的传感器测量通过链脲佐菌素给药制备的II型糖尿病大鼠的血液山梨糖醇水平。山梨糖醇的值与食品分析的F-kit测得的值高度相关，并且我们证实了山梨糖醇的浓度可以使用开发的生物传感器进行定量。此外，醛糖还原酶抑制剂“eparlrestat”，这是一种抑制山梨糖醇积累的治疗药物，给糖尿病大鼠服用，用开发的生物传感器测量血液中的山梨醇水平。因此,治疗组和非治疗组的血糖水平均较高，但治疗组的血液山梨糖醇水平下降。结果表明，除了血糖水平外，使用开发的生物传感器测量山梨糖醇水平还可以评估并发症，例如糖尿病性神经病变。在未来,我们预计开发的山梨醇生物传感器将小型化，将简化血液样本的预处理方法，并将其应用于糖尿病并发症治疗剂的开发和个性化医疗。
