PDF(Pubmed)
Abstract：
In contrast to the well-characterized gut microbiomes, the composition and function of the insect body-surface microbiotas are still elusive and highly underexplored. Here we report the dynamic features of the Drosophila melanogaster surface microbiomes. It was found that the microbiomes assembled on fly surfaces could defend insects against fungal parasitic infections. The substantial increase of bacterial loads occurred within 10 days of fly eclosion, especially the expansion of Gilliamella species. The culturable bacteria such as Lactiplantibacillus plantarum could effectively inhibit fungal spore germinations, and the gnotobiotic addition of the isolated bacteria could substantially delay fungal infection of axenic flies. We found that the fly tarsal segments were largely accumulated with bacterial cells, which could accelerate cell dispersal onto different body parts to deter fungal spore germinations. Our findings will facilitate future investigations of the surface microbiotas affecting insect physiologies.
摘要：
与特征明确的肠道微生物组相反，昆虫体表微生物的组成和功能仍然难以捉摸，并且开发不足。在这里，我们报告了果蝇表面微生物组的动态特征。发现在果蝇表面组装的微生物组可以防御昆虫免受真菌寄生虫感染。细菌载量的大幅增加发生在苍蝇羽化后的10天内，特别是Gilliamella物种的扩张。植物乳杆菌等可培养细菌能有效抑制真菌孢子萌发,并且分离的细菌的微生物添加可以大大延迟无菌果蝇的真菌感染。我们发现，蝇tar骨大部分是细菌细胞积累的，这可以加速细胞扩散到不同的身体部位，以阻止真菌孢子发芽。我们的发现将有助于将来研究影响昆虫生理的表面微生物。
