PDF(Pubmed)
Abstract：
Pre-eclampsia has remained an elusive disease with serious impacts on both maternal and foetal health. Two novel markers, annexin A5 (ANXA5) and apelin are currently of considerable interest. The present study aimed to determine the placental expression of ANXA5 and apelin in pre-eclamptic placentae and also to elucidate if there is any correlation between the expression of these markers and the clinical features of both, mother and neonate. The comparison between gross and histopathological features of pre-eclamptic placentae and controls was another objective.
A prospective, observational study was undertaken for one year. Placentae of pre-eclamptic patients and matched controls (matched for age, ethnic and socio-economic background) were collected along with the clinical data. Gross and histopathological analyses were done and immunohistochemical study of placental sections with ANXA5 and apelin was also undertaken.
79 pre-eclamptic patients and equal numbers of matched controls were included in the study. The difference in weight and dimensions of placentae between the pre-eclampsia group and matched controls was significant. Histopathological features noted in the pre-eclamptic placentae included decidual vasculopathy, infarction, perivillous fibrin deposition, increased syncytial knots and distal villous hypoplasia. There was a significant reduction in the expression of both ANXA5 and apelin in pre-eclamptic placentae compared to controls. Among pre-eclamptic patients, the intensity of ANXA5 and apelin expression showed a significant association with respect to neonatal resuscitation. Furthermore, the intensity of apelin showed expression a significant correlation with the requirement of sick neonatal care unit treatment.
The results of the present study suggest that both ANXA5 and apelin levels are reduced in pre-eclamptic placentae. Hence, it is recommended to further explore the impact of these markers on pregnancy outcomes by undertaking randomized controlled trials.
A prospective, observational study was undertaken for one year. Placentae of pre-eclamptic patients and matched controls (matched for age, ethnic and socio-economic background) were collected along with the clinical data. Gross and histopathological analyses were done and immunohistochemical study of placental sections with ANXA5 and apelin was also undertaken.
79 pre-eclamptic patients and equal numbers of matched controls were included in the study. The difference in weight and dimensions of placentae between the pre-eclampsia group and matched controls was significant. Histopathological features noted in the pre-eclamptic placentae included decidual vasculopathy, infarction, perivillous fibrin deposition, increased syncytial knots and distal villous hypoplasia. There was a significant reduction in the expression of both ANXA5 and apelin in pre-eclamptic placentae compared to controls. Among pre-eclamptic patients, the intensity of ANXA5 and apelin expression showed a significant association with respect to neonatal resuscitation. Furthermore, the intensity of apelin showed expression a significant correlation with the requirement of sick neonatal care unit treatment.
The results of the present study suggest that both ANXA5 and apelin levels are reduced in pre-eclamptic placentae. Hence, it is recommended to further explore the impact of these markers on pregnancy outcomes by undertaking randomized controlled trials.
摘要：
先兆子痫仍然是一种难以捉摸的疾病，对孕产妇和胎儿健康都有严重影响。两个新颖的标记，附件A5（ANXA5）和apelin目前引起了人们的极大兴趣。本研究旨在确定子痫前期胎盘中ANXA5和apelin的表达，并阐明这些标志物的表达与两者的临床特征之间是否存在任何相关性。母亲和新生儿。子痫前期胎盘和对照的总体和组织病理学特征之间的比较是另一个目标。
有前景的,观察性研究为期一年.先兆子痫患者的胎盘和匹配的对照(年龄匹配，种族和社会经济背景)与临床数据一起收集。进行了大体和组织病理学分析，并对ANXA5和apelin的胎盘切片进行了免疫组织化学研究。
研究中包括79名先兆子痫患者和相同数量的匹配对照。子痫前期组和匹配对照组之间胎盘的重量和尺寸差异显着。先兆子痫胎盘的组织病理学特征包括蜕膜血管病变，梗塞,绒毛周围纤维蛋白沉积，合胞体结增加和远端绒毛发育不全。与对照相比,在先兆子痫胎盘中ANXA5和apelin的表达均显著降低。在先兆子痫患者中，ANXA5和apelin的表达强度与新生儿复苏有显著相关性.此外，apelin的表达强度与患病新生儿监护病房治疗的需求显着相关。
本研究的结果表明，先兆子痫胎盘中ANXA5和apelin水平均降低。因此，建议通过开展随机对照试验,进一步探讨这些标志物对妊娠结局的影响.
有前景的,观察性研究为期一年.先兆子痫患者的胎盘和匹配的对照(年龄匹配，种族和社会经济背景)与临床数据一起收集。进行了大体和组织病理学分析，并对ANXA5和apelin的胎盘切片进行了免疫组织化学研究。
研究中包括79名先兆子痫患者和相同数量的匹配对照。子痫前期组和匹配对照组之间胎盘的重量和尺寸差异显着。先兆子痫胎盘的组织病理学特征包括蜕膜血管病变，梗塞,绒毛周围纤维蛋白沉积，合胞体结增加和远端绒毛发育不全。与对照相比,在先兆子痫胎盘中ANXA5和apelin的表达均显著降低。在先兆子痫患者中，ANXA5和apelin的表达强度与新生儿复苏有显著相关性.此外，apelin的表达强度与患病新生儿监护病房治疗的需求显着相关。
本研究的结果表明，先兆子痫胎盘中ANXA5和apelin水平均降低。因此，建议通过开展随机对照试验,进一步探讨这些标志物对妊娠结局的影响.
