A prospective, observational study was undertaken for one year. Placentae of pre-eclamptic patients and matched controls (matched for age, ethnic and socio-economic background) were collected along with the clinical data. Gross and histopathological analyses were done and immunohistochemical study of placental sections with ANXA5 and apelin was also undertaken.
79 pre-eclamptic patients and equal numbers of matched controls were included in the study. The difference in weight and dimensions of placentae between the pre-eclampsia group and matched controls was significant. Histopathological features noted in the pre-eclamptic placentae included decidual vasculopathy, infarction, perivillous fibrin deposition, increased syncytial knots and distal villous hypoplasia. There was a significant reduction in the expression of both ANXA5 and apelin in pre-eclamptic placentae compared to controls. Among pre-eclamptic patients, the intensity of ANXA5 and apelin expression showed a significant association with respect to neonatal resuscitation. Furthermore, the intensity of apelin showed expression a significant correlation with the requirement of sick neonatal care unit treatment.
The results of the present study suggest that both ANXA5 and apelin levels are reduced in pre-eclamptic placentae. Hence, it is recommended to further explore the impact of these markers on pregnancy outcomes by undertaking randomized controlled trials.
有前景的,观察性研究为期一年.先兆子痫患者的胎盘和匹配的对照(年龄匹配,种族和社会经济背景)与临床数据一起收集。进行了大体和组织病理学分析,并对ANXA5和apelin的胎盘切片进行了免疫组织化学研究。
研究中包括79名先兆子痫患者和相同数量的匹配对照。子痫前期组和匹配对照组之间胎盘的重量和尺寸差异显着。先兆子痫胎盘的组织病理学特征包括蜕膜血管病变,梗塞,绒毛周围纤维蛋白沉积,合胞体结增加和远端绒毛发育不全。与对照相比,在先兆子痫胎盘中ANXA5和apelin的表达均显著降低。在先兆子痫患者中,ANXA5和apelin的表达强度与新生儿复苏有显著相关性.此外,apelin的表达强度与患病新生儿监护病房治疗的需求显着相关。
本研究的结果表明,先兆子痫胎盘中ANXA5和apelin水平均降低。因此,建议通过开展随机对照试验,进一步探讨这些标志物对妊娠结局的影响.