PDF(Pubmed)
Abstract：
High-salt diet (HSD) is associated with dysregulated metabolism and metabolic disorders. Although previous studies have indicated its effect on metabolic tissues, the involving molecular mechanisms are not quite understood. In the present study, we provided a comprehensive transcriptome analysis on multiple metabolic tissues of HSD-fed mouse model by RNA sequencing. We observed that several genes associated with de novo lipogenesis and cholesterol biosynthesis were significantly downregulated in white adipose tissue and liver tissue of HSD mice group, such as Fasn, Scd1, Acaca, and Thrsp. Furthermore, combined with secretome datasets, our results further demonstrated that HSD could alter expression levels of organokines in metabolic tissues, for example, Tsk and Manf, in liver tissue and, thus, possibly mediate cross-talk between different metabolic tissues. Our study provided new insight about molecular signatures of HSD on multiple metabolic tissues.
摘要：
高盐饮食（HSD）与代谢失调和代谢紊乱有关。尽管先前的研究表明其对代谢组织的影响，涉及的分子机制还不太清楚。在本研究中,我们通过RNA测序对HSD饲喂小鼠模型的多个代谢组织进行了全面的转录组分析。我们观察到HSD小鼠组的白色脂肪组织和肝脏组织中几个与从头脂肪生成和胆固醇生物合成相关的基因显著下调。比如Fasn,Scd1,Acaca,和Thrsp。此外，结合分泌组数据集，我们的结果进一步证明，HSD可以改变代谢组织中有机因子的表达水平，例如,Tsk和Manf,在肝脏组织中，因此，可能介导不同代谢组织之间的串扰。我们的研究提供了有关HSD在多种代谢组织上的分子特征的新见解。
