PDF(Pubmed)
Abstract：
Emerging evidence points to coordinated action of chemical and mechanical cues during brain development. At early stages of neocortical development, angiogenic factors and chemokines such as CXCL12, ephrins, and semaphorins assume crucial roles in orchestrating neuronal migration and axon elongation of postmitotic neurons. Here we explore the intrinsic mechanical properties of the developing marginal zone of the pallium in the migratory pathways and brain distribution of the pioneer Cajal-Retzius cells. These neurons are generated in several proliferative regions in the developing brain (e.g., the cortical hem and the pallial subpallial boundary) and migrate tangentially in the preplate/marginal zone covering the upper portion of the developing cortex. These cells play crucial roles in correct neocortical layer formation by secreting several molecules such as Reelin. Our results indicate that the motogenic properties of Cajal-Retzius cells and their perinatal distribution in the marginal zone are modulated by both chemical and mechanical factors, by the specific mechanical properties of Cajal-Retzius cells, and by the differential stiffness of the migratory routes. Indeed, cells originating in the cortical hem display higher migratory capacities than those generated in the pallial subpallial boundary which may be involved in the differential distribution of these cells in the dorsal-lateral axis in the developing marginal zone.
摘要：
新出现的证据表明，大脑发育过程中化学和机械线索的协调作用。在新皮质发育的早期阶段，血管生成因子和趋化因子，如CXCL12，ephrins，和信号蛋白在协调有丝分裂后神经元的神经元迁移和轴突伸长中起着至关重要的作用。在这里，我们探索了先驱Cajal-Retzius细胞的迁移途径和脑分布中大脑皮层发育边缘区的内在机械特性。这些神经元在发育中的大脑中的几个增殖区域产生（例如，皮质下摆和骨膜下边界），并在覆盖发育中的皮质上部的预板/边缘区域中切向迁移。这些细胞通过分泌几种分子如Reelin在正确的新皮质层形成中起关键作用。我们的结果表明，Cajal-Retzius细胞的运动特性及其在边缘区的围产期分布受化学和机械因素的调节，通过Cajal-Retzius细胞的特定机械性能，以及迁移路线的不同刚度。的确,起源于皮质下摆的细胞显示出比在pallial-pallial下边界产生的细胞更高的迁移能力，这可能与这些细胞在发育边缘区的背外侧轴中的差异分布有关。
