Abstract：
MicroRNAs (miRNAs) are small non-coding RNAs that participate in various biological and cellular processes by regulating target gene expression. miRNAs are also known to play vital roles in the pathogenesis of various diseases, including infections, as well as the disease progression and defense responses. In this study, we examined the expression levels of pol-miR-140-3p and its target gene, kinesin family member 5A (KIF5A), in association with the Streptococcus parauberis (S. parauberis) infection, a major bacterial pathogen that causes streptococcosis in olive flounder (Paralichthys olivaceus). KIF5A is a heavy chain isoform of kinesin-1, which is known to be brain-specific, and this study is the first examination of KIF5A expression related to the regulation of miRNA in olive flounder (named PoKIF5A). There were significant differences in expression levels between infected and healthy olive flounder as the expression of pol-miR-140-3p in the infected fish was lower than that in the control, while the expression of PoKIF5A was higher in the infected fish than in the healthy controls. These contradictory results suggest that downregulated pol-miR-140-3p induces the expression of PoKIF5A against S. parauberis infection in olive flounder.
摘要：
MicroRNAs(miRNA)是通过调控靶基因表达参与各种生物和细胞过程的非编码小RNA。miRNA也被认为在各种疾病的发病机制中起着至关重要的作用。包括感染,以及疾病进展和防御反应。在这项研究中,我们检测了pol-miR-140-3p及其靶基因的表达水平，驱动蛋白家族成员5A（KIF5A），与副链球菌相关(S.旁瓣)感染，一种引起比目鱼链球菌病的主要细菌病原体。KIF5A是驱动蛋白-1的重链同工型，已知是脑特异性的，本研究首次考察了橄榄比目鱼(命名为PoKIF5A)中KIF5A表达调控相关的miRNA。受感染的比目鱼和健康比目鱼之间的表达水平存在显着差异，因为受感染的鱼中pol-miR-140-3p的表达低于对照，而PoKIF5A在感染鱼中的表达高于健康对照。这些矛盾的结果表明，下调的pol-miR-140-3p诱导了PoKIF5A在橄榄比目鱼中的表达。
