PDF(Pubmed)
Abstract：
UNASSIGNED: Collagen protein plays a notable role maintaining firm skin. Topical creams containing collagen fibers are widely available, but their usefulness is questionable due to limited skin penetration. When applied in a cream, collagen does not penetrate the skin leaving the skin structure unaffected.
UNASSIGNED: We formulated micronized collagen in a cream base. Using human skin samples, we sought to investigate the ability of the micronized collagen cream to penetrate human skin.
UNASSIGNED: Particle sizes of micronized marine collagen were evaluated using electron microscopy. Optical profilometry was conducted to evaluate skin topography and roughness. The antioxidant activity of the collagen was evaluated using the electron paramagnetic resonance technique by measuring the changes in free radical production. Collagen penetration depth in human skin samples was monitored using a non-invasive optical technique known as iterative multiplane optical property extraction, which works based on the detection of laser light phase changes following the presence of collagen particles in deep skin layers.
UNASSIGNED: According to the electron microscopy, collagen particles were found to be of various sizes, the smallest being about 120nm in diameter. Skin topography measurements revealed that the treated collagen cream increased skin smoothness of the samples. Our results derived from the iterative multiplane optical property extraction indicated that micronized collagen in a cream base penetrates both the stratum corneum and the deep epidermal layers toward the dermis.
UNASSIGNED: Our investigation suggests that the collagen in the studied cream formulation was able to penetrate the stratum coreum and deep epidermal layers in human skin samples.
UNASSIGNED: We formulated micronized collagen in a cream base. Using human skin samples, we sought to investigate the ability of the micronized collagen cream to penetrate human skin.
UNASSIGNED: Particle sizes of micronized marine collagen were evaluated using electron microscopy. Optical profilometry was conducted to evaluate skin topography and roughness. The antioxidant activity of the collagen was evaluated using the electron paramagnetic resonance technique by measuring the changes in free radical production. Collagen penetration depth in human skin samples was monitored using a non-invasive optical technique known as iterative multiplane optical property extraction, which works based on the detection of laser light phase changes following the presence of collagen particles in deep skin layers.
UNASSIGNED: According to the electron microscopy, collagen particles were found to be of various sizes, the smallest being about 120nm in diameter. Skin topography measurements revealed that the treated collagen cream increased skin smoothness of the samples. Our results derived from the iterative multiplane optical property extraction indicated that micronized collagen in a cream base penetrates both the stratum corneum and the deep epidermal layers toward the dermis.
UNASSIGNED: Our investigation suggests that the collagen in the studied cream formulation was able to penetrate the stratum coreum and deep epidermal layers in human skin samples.
摘要：
■胶原蛋白在保持皮肤紧致方面起着显著的作用。含有胶原纤维的外用乳膏广泛可用，但是由于皮肤渗透有限，它们的有用性值得怀疑。当应用在乳霜中时，胶原蛋白不渗透皮肤，使皮肤结构不受影响。
■我们在乳膏基质中配制了微粉化的胶原蛋白。使用人体皮肤样本，我们试图研究微粉化胶原蛋白霜渗透人体皮肤的能力。
■使用电子显微镜评估微粉化海洋胶原的粒度。进行光学轮廓测量以评估皮肤形貌和粗糙度。使用电子顺磁共振技术通过测量自由基产生的变化来评估胶原蛋白的抗氧化活性。使用称为迭代多平面光学性质提取的非侵入性光学技术监测人体皮肤样品中的胶原蛋白穿透深度，它的工作原理是在深层皮肤中存在胶原蛋白颗粒后检测激光相位变化。
■根据电子显微镜，胶原蛋白颗粒被发现有不同的大小，最小的直径约为120nm。皮肤形貌测量显示,经处理的胶原乳膏增加了样品的皮肤光滑度。我们从迭代多平面光学特性提取得出的结果表明，乳膏基质中的微粉化胶原蛋白会向真皮渗透角质层和深层表皮层。
我们的研究表明,所研究的乳膏制剂中的胶原蛋白能够渗透人皮肤样品中的芯层和深表皮层。
■我们在乳膏基质中配制了微粉化的胶原蛋白。使用人体皮肤样本，我们试图研究微粉化胶原蛋白霜渗透人体皮肤的能力。
■使用电子显微镜评估微粉化海洋胶原的粒度。进行光学轮廓测量以评估皮肤形貌和粗糙度。使用电子顺磁共振技术通过测量自由基产生的变化来评估胶原蛋白的抗氧化活性。使用称为迭代多平面光学性质提取的非侵入性光学技术监测人体皮肤样品中的胶原蛋白穿透深度，它的工作原理是在深层皮肤中存在胶原蛋白颗粒后检测激光相位变化。
■根据电子显微镜，胶原蛋白颗粒被发现有不同的大小，最小的直径约为120nm。皮肤形貌测量显示,经处理的胶原乳膏增加了样品的皮肤光滑度。我们从迭代多平面光学特性提取得出的结果表明，乳膏基质中的微粉化胶原蛋白会向真皮渗透角质层和深层表皮层。
我们的研究表明,所研究的乳膏制剂中的胶原蛋白能够渗透人皮肤样品中的芯层和深表皮层。
