Abstract：
Sucrose, one of the most widespread disaccharides in nature, has been available in daily human life for many centuries. As an abundant and cheap sweetener, sucrose plays an essential role in our diet and the food industry. However, it has been determined that many diseases, such as obesity, diabetes, hyperlipidemia, etc., directly relate to the overconsumption of sucrose. It arouses many explorations for the conversion of sucrose to high-value chemicals. Production of valuable substances from sucrose by chemical methods has been studied since a half-century ago. Compared to chemical processes, biotechnological conversion approaches of sucrose are more environmentally friendly. Many enzymes can use sucrose as the substrate to generate functional sugars, especially those from GH68, GH70, GH13, and GH32 families. In this review, enzymatic catalysis and whole-cell fermentation of sucrose for the production of valuable chemicals were reviewed. The multienzyme cascade catalysis and metabolic engineering strategies were addressed.
摘要：
蔗糖,自然界中最普遍的二糖之一，在人类日常生活中已经存在了许多世纪。作为一种丰富而廉价的甜味剂，蔗糖在我们的饮食和食品工业中起着至关重要的作用。然而,已经确定许多疾病，比如肥胖，糖尿病,高脂血症,等。,直接关系到蔗糖的过度消耗。它引起了许多探索蔗糖转化为高价值化学品。自半个世纪以来，人们一直在研究通过化学方法从蔗糖生产有价值的物质。与化学过程相比，蔗糖的生物技术转化方法更环保。许多酶可以使用蔗糖作为底物来产生功能性糖，特别是来自GH68、GH70、GH13和GH32家族的。在这次审查中,综述了蔗糖的酶催化和全细胞发酵生产有价值化学品的方法。讨论了多酶级联催化和代谢工程策略。
