Abstract：
The tumor microenvironment (TME) and the host inflammatory response are closely interconnected. The interplay between systemic inflammation and the local immune response may influence tumor development and progression in various types of cancer. The systemic immune-inflammation index (SII) represents a prognostic marker for germ cell tumors (GCTs). The aim of the present study was to detect specific immune cell subpopulation changes which were associated with the SII level in chemotherapy-naïve GCT patients. In total, 51 GCT patients, prior to cisplatin-based chemotherapy, were included in the present study. Immunophenotyping of peripheral blood leukocyte subpopulations was performed using flow cytometry. The SII level was correlated with the percentage of various leukocyte subpopulations. The obtained results demonstrated that SII levels above the cut-off value of SII ≥ 1003 were associated with higher neutrophil percentages. An inverse correlation was found between the SII and the peripheral lymphocyte percentage that logically reflects the calculations of the SII index. Furthermore, the presented data also showed that in the lymphocyte subpopulation, the association with the SII was driven by T-cell subpopulations. In innate immunity-cell subpopulations, we observed a correlation between SII level and neutrophils as well as associations with eosinophil, basophil, natural killer cell and dendritic cell percentages. We suppose that the described interactions represent a manifestation of cancer-induced immune suppression. The results of the present study contribute to the elucidation of the interrelationship between tumor cells and the innate/adaptive immune system of the host.
摘要：
肿瘤微环境(TME)与宿主炎症反应密切相关。全身性炎症和局部免疫反应之间的相互作用可能会影响各种类型癌症中的肿瘤发展和进展。全身免疫炎症指数（SII）代表生殖细胞肿瘤（GCT）的预后标志物。本研究的目的是检测与化疗初治GCT患者SII水平相关的特异性免疫细胞亚群变化。总的来说,51例GCT患者，在以顺铂为基础的化疗之前，包括在本研究中。使用流式细胞术进行外周血白细胞亚群的免疫表型分型。SII水平与各种白细胞亚群的百分比相关。获得的结果表明，高于SII≥1003的临界值的SII水平与较高的中性粒细胞百分比相关。在SII和外周淋巴细胞百分比之间发现了负相关，这在逻辑上反映了SII指数的计算。此外，所提供的数据还表明，在淋巴细胞亚群中，与SII的关联由T细胞亚群驱动.在先天免疫细胞亚群中，我们观察到SII水平与嗜中性粒细胞之间的相关性以及与嗜酸性粒细胞的相关性，嗜碱性粒细胞,自然杀伤细胞和树突状细胞百分比。我们认为所描述的相互作用代表了癌症诱导的免疫抑制的表现。本研究的结果有助于阐明肿瘤细胞与宿主的先天/适应性免疫系统之间的相互关系。
