PDF(Pubmed)
Abstract：
UNASSIGNED: Angiogenesis plays a critical role in the growth and metastasis of breast cancer and angiogenesis inhibition has become an effective strategy for cancer therapy. Our study aimed to clarify the key candidate genes and pathways related to breast cancer angiogenesis.
UNASSIGNED: Differentially expressed genes (DEGs) in the raw breast cancer (BRCA) gene dataset from the Cancer Genome Atlas (TCGA) database were identified and gene ontology analysis of the DEGs was performed. Hub genes were subsequently determined using the Gene Expression Omnibus database. The expression of the mesenchyme homeobox 2 (MEOX2) in breast cancer cells and tissues was assessed by quantification real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC), respectively. The prognostic value of the MEOX2 gene in breast cancer tissue was evaluated with the Kaplan-Meier plotter.
UNASSIGNED: A total of 61 angiogenesis-related DEGs were identified in the TCGA dataset, among which the gene MEOX2 was significantly down-regulated. GO functional annotation and pathway enrichment analyses showed that MEOX2 was significantly enriched in the regulation of vasculature development. The IHC results confirmed that MEOX2 expression was repressed in breast cancer tissues and the relatively low level indicated the tissue was densely vascularized. Moreover, MEOX2 expression was significantly elevated in breast cancer cells after treatment with cisplatin (DDP) and epirubicin (EPI). Finally, the Kaplan-Meier plotter confirmed that higher expression levels of MEOX2 were related to better overall survival.
UNASSIGNED: Our study revealed that the angiogenesis-associated gene MEOX2 can be used as a novel biomarker for breast cancer diagnosis and clinical therapy.
UNASSIGNED: Differentially expressed genes (DEGs) in the raw breast cancer (BRCA) gene dataset from the Cancer Genome Atlas (TCGA) database were identified and gene ontology analysis of the DEGs was performed. Hub genes were subsequently determined using the Gene Expression Omnibus database. The expression of the mesenchyme homeobox 2 (MEOX2) in breast cancer cells and tissues was assessed by quantification real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC), respectively. The prognostic value of the MEOX2 gene in breast cancer tissue was evaluated with the Kaplan-Meier plotter.
UNASSIGNED: A total of 61 angiogenesis-related DEGs were identified in the TCGA dataset, among which the gene MEOX2 was significantly down-regulated. GO functional annotation and pathway enrichment analyses showed that MEOX2 was significantly enriched in the regulation of vasculature development. The IHC results confirmed that MEOX2 expression was repressed in breast cancer tissues and the relatively low level indicated the tissue was densely vascularized. Moreover, MEOX2 expression was significantly elevated in breast cancer cells after treatment with cisplatin (DDP) and epirubicin (EPI). Finally, the Kaplan-Meier plotter confirmed that higher expression levels of MEOX2 were related to better overall survival.
UNASSIGNED: Our study revealed that the angiogenesis-associated gene MEOX2 can be used as a novel biomarker for breast cancer diagnosis and clinical therapy.
摘要：
未经证实：血管生成在乳腺癌的生长和转移中起关键作用，抑制血管生成已成为癌症治疗的有效策略。我们的研究旨在阐明与乳腺癌血管生成相关的关键候选基因和途径。
UNASSIGNED:鉴定来自癌症基因组图谱(TCGA)数据库的原始乳腺癌(BRCA)基因数据集中的差异表达基因(DEGs),并进行DEGs的基因本体论分析。随后使用基因表达综合数据库确定Hub基因。通过定量实时聚合酶链反应（qRT-PCR）和免疫组织化学（IHC）评估了间充质同源盒2（MEOX2）在乳腺癌细胞和组织中的表达，分别。用Kaplan-Meier绘图仪评价MEOX2基因在乳腺癌组织中的预后价值。
未经证实：在TCGA数据集中总共鉴定出61个血管生成相关的DEGs，其中MEOX2基因显著下调。GO功效注释和通路富集剖析显示，MEOX2在调控血管发育方面显著富集。IHC结果证实MEOX2表达在乳腺癌组织中被抑制,并且相对低水平表明该组织是密集血管化的。此外，用顺铂（DDP）和表柔比星（EPI）治疗后，乳腺癌细胞中MEOX2的表达显着升高。最后,Kaplan-Meier绘图仪证实,较高的MEOX2表达水平与较好的总生存期相关.
UNASSIGNED：我们的研究表明，血管生成相关基因MEOX2可作为乳腺癌诊断和临床治疗的新型生物标志物。
UNASSIGNED:鉴定来自癌症基因组图谱(TCGA)数据库的原始乳腺癌(BRCA)基因数据集中的差异表达基因(DEGs),并进行DEGs的基因本体论分析。随后使用基因表达综合数据库确定Hub基因。通过定量实时聚合酶链反应（qRT-PCR）和免疫组织化学（IHC）评估了间充质同源盒2（MEOX2）在乳腺癌细胞和组织中的表达，分别。用Kaplan-Meier绘图仪评价MEOX2基因在乳腺癌组织中的预后价值。
未经证实：在TCGA数据集中总共鉴定出61个血管生成相关的DEGs，其中MEOX2基因显著下调。GO功效注释和通路富集剖析显示，MEOX2在调控血管发育方面显著富集。IHC结果证实MEOX2表达在乳腺癌组织中被抑制,并且相对低水平表明该组织是密集血管化的。此外，用顺铂（DDP）和表柔比星（EPI）治疗后，乳腺癌细胞中MEOX2的表达显着升高。最后,Kaplan-Meier绘图仪证实,较高的MEOX2表达水平与较好的总生存期相关.
UNASSIGNED：我们的研究表明，血管生成相关基因MEOX2可作为乳腺癌诊断和临床治疗的新型生物标志物。
