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Abstract：
UNASSIGNED: Traditional morphological imaging of intervertebral disc herniation (IVDH) is challenging in early disease diagnosis. Aiming at the early diagnosis of IVD by non-invasive molecular imaging targeting of integrin α5β1, we performed novel imaging in rats with acute IVDH for the first time.
UNASSIGNED: Animal models were prepared by conducting an established needle puncture procedure through the normal intervertebral disc (IVD). The disc-injured rats underwent SPECT/CT imaging of the 99mTc-3PisoDGR2 peptide at 1 day to 2 months postinjury. The expression change of integrin α5β1 was determined by anti-integrin α5 and anti-integrin α5β1 immunohistochemistry (IHC). Magnetic resonance imaging (MRI) was performed for comparison during disease progression. The morphological changes of the disc were determined by safranin-O staining.
UNASSIGNED: Rats with acute IVDH showed gradually increased disc uptake of 99mTc-3PisoDGR2 from 1 to 7 days posttreatment, which was a significantly higher level than that of the normal disks in degenerative diseases. IHC results showed the expression of integrin α5β1 on the surface of annulus fibrosus (AF) cells and nucleus pulposus (NP) cells, which agreed with the uptake data. MRI showed a progressively decreased T2 density and MRI index throughout the investigation. Hematoxylin and eosin (HE) staining and safranin-O staining revealed a disorganized structure of the IVD as well as loss of proteoglycans after puncture.
UNASSIGNED: The present study demonstrated a good correlation between integrin α5β1 expression and acute disc herniation. The SPECT/CT imaging of 99mTc-3PisoDGR2 targeting integrin α5β1 may diagnose IVDH in an acute phase for early disease management.
UNASSIGNED: Animal models were prepared by conducting an established needle puncture procedure through the normal intervertebral disc (IVD). The disc-injured rats underwent SPECT/CT imaging of the 99mTc-3PisoDGR2 peptide at 1 day to 2 months postinjury. The expression change of integrin α5β1 was determined by anti-integrin α5 and anti-integrin α5β1 immunohistochemistry (IHC). Magnetic resonance imaging (MRI) was performed for comparison during disease progression. The morphological changes of the disc were determined by safranin-O staining.
UNASSIGNED: Rats with acute IVDH showed gradually increased disc uptake of 99mTc-3PisoDGR2 from 1 to 7 days posttreatment, which was a significantly higher level than that of the normal disks in degenerative diseases. IHC results showed the expression of integrin α5β1 on the surface of annulus fibrosus (AF) cells and nucleus pulposus (NP) cells, which agreed with the uptake data. MRI showed a progressively decreased T2 density and MRI index throughout the investigation. Hematoxylin and eosin (HE) staining and safranin-O staining revealed a disorganized structure of the IVD as well as loss of proteoglycans after puncture.
UNASSIGNED: The present study demonstrated a good correlation between integrin α5β1 expression and acute disc herniation. The SPECT/CT imaging of 99mTc-3PisoDGR2 targeting integrin α5β1 may diagnose IVDH in an acute phase for early disease management.
摘要：
UNASSIGNED：椎间盘突出症（IVDH）的传统形态学成像在早期疾病诊断中具有挑战性。针对以整合素α5β1为靶点的非侵入性分子影像学对IVD的早期诊断,首次对急性IVDH大鼠进行了新的影像学检查。
UNASSIGNED:通过对正常椎间盘(IVD)进行既定的针穿刺程序来制备动物模型。椎间盘损伤大鼠在损伤后1天至2个月接受99mTc-3PisoDGR2肽的SPECT/CT成像。通过抗整合素α5和抗整合素α5β1免疫组织化学（IHC）确定整合素α5β1的表达变化。在疾病进展期间进行磁共振成像（MRI）进行比较。通过savranin-O染色确定椎间盘的形态变化。
UNASSIGNED：急性IVDH大鼠在治疗后1至7天显示99mTc-3PisoDGR2的椎间盘摄取逐渐增加，在退行性疾病中明显高于正常椎间盘。免疫组化结果显示整合素α5β1在纤维环(AF)细胞和髓核(NP)细胞表面表达,这与吸收数据一致。在整个研究过程中，MRI显示T2密度和MRI指数逐渐降低。苏木精和伊红（HE）染色和番红-O染色显示IVD的结构混乱，穿刺后蛋白聚糖丢失。
未经证实：本研究表明整合素α5β1表达与急性椎间盘突出症之间存在良好的相关性。99mTc-3PisoDGR2靶向整合素α5β1的SPECT/CT成像可在急性期诊断IVDH，用于早期疾病管理。
UNASSIGNED:通过对正常椎间盘(IVD)进行既定的针穿刺程序来制备动物模型。椎间盘损伤大鼠在损伤后1天至2个月接受99mTc-3PisoDGR2肽的SPECT/CT成像。通过抗整合素α5和抗整合素α5β1免疫组织化学（IHC）确定整合素α5β1的表达变化。在疾病进展期间进行磁共振成像（MRI）进行比较。通过savranin-O染色确定椎间盘的形态变化。
UNASSIGNED：急性IVDH大鼠在治疗后1至7天显示99mTc-3PisoDGR2的椎间盘摄取逐渐增加，在退行性疾病中明显高于正常椎间盘。免疫组化结果显示整合素α5β1在纤维环(AF)细胞和髓核(NP)细胞表面表达,这与吸收数据一致。在整个研究过程中，MRI显示T2密度和MRI指数逐渐降低。苏木精和伊红（HE）染色和番红-O染色显示IVD的结构混乱，穿刺后蛋白聚糖丢失。
未经证实：本研究表明整合素α5β1表达与急性椎间盘突出症之间存在良好的相关性。99mTc-3PisoDGR2靶向整合素α5β1的SPECT/CT成像可在急性期诊断IVDH，用于早期疾病管理。
